Lilley Kathryn S, Dupree Paul
Department of Biochemistry, University of Cambridge, Building 0, Downing Site, Cambridge CB2 1QW, UK.
J Exp Bot. 2006;57(7):1493-9. doi: 10.1093/jxb/erj141.
Many cell biologists wish to know the subcellular localization of proteins of interest. Proteomics methods have the potential to describe the entire protein content of organelles. However, practical limitations in organelle isolation and analysis of low abundance proteins have meant that organelle proteomics has had, until recently, only limited success. Some examples of quantitative proteomic methods and their use in the study of plant organelle proteomes are discussed here. It is concluded that 2D-difference gel electrophoresis (2D-DIGE) as well as differential isotope tagging strategies coupled to non-gel-based LC-MS are proving useful in this area of research.
许多细胞生物学家希望了解感兴趣蛋白质的亚细胞定位。蛋白质组学方法有潜力描述细胞器的全部蛋白质组成。然而，细胞器分离和低丰度蛋白质分析中的实际限制意味着直到最近，细胞器蛋白质组学仅取得了有限的成功。本文讨论了定量蛋白质组学方法的一些实例及其在植物细胞器蛋白质组研究中的应用。得出的结论是，二维差异凝胶电泳（2D-DIGE）以及与非凝胶基液相色谱-质谱联用的差异同位素标记策略在该研究领域已证明是有用的。
