蛋白质印迹免疫测定法作为猪血清中抗猪肺炎支原体抗体存在的确认试验。
Western blot immunoassay as a confirmatory test for the presence of anti-Mycoplasma hyopneumoniae antibodies in swine serum.
作者信息
Ameri Mehrdad, Zhou En-Min, Hsu Walter H
机构信息
Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames 50011, USA.
出版信息
J Vet Diagn Invest. 2006 Mar;18(2):198-201. doi: 10.1177/104063870601800210.
A Western blot immunoassay (WBI) was developed as a confirmatory test for 2 commercial Mycoplasma hyopneumoniae (Mhyo) ELISAs. The WBI detected at least 5 antigen bands (150, 130, 74, 70, and 30 kDa) from Mhyo whole membrane proteins that were not present in the antigens prepared from M. hyorhinis and M. hyosynoviae. Among discrepant sera from vaccinated pigs (n = 17) and field samples (n = 91) assayed by WBI: 1) 2 of the ELISA-positive samples reacted with all 5 antigens bands; 2) all blocking ELISA-positive samples (n = 53) bound 150-, 130-, 74-, and 70-kD antigen bands; and 3) all indirect ELISA-positive samples (n = 55) bound 150-, 130-, and 30-kD antigens. We conclude that the WBI targeting the top 4 antigen bands is a useful confirmatory test for samples initially screened using the commercial Mhyo ELISAs.
开发了一种蛋白质免疫印迹免疫测定法（WBI）作为两种市售猪肺炎支原体（Mhyo）酶联免疫吸附测定（ELISA）的确认试验。WBI检测到来自猪肺炎支原体全膜蛋白的至少5条抗原带（150、130、74、70和30 kDa），而这些抗原带在从猪鼻支原体和猪滑膜支原体制备的抗原中不存在。在通过WBI检测的接种疫苗猪的差异血清（n = 17）和田间样本（n = 91）中：1）2个ELISA阳性样本与所有5条抗原带发生反应；2）所有阻断ELISA阳性样本（n = 53）均结合150、130、74和70 kDa抗原带；3）所有间接ELISA阳性样本（n = 55）均结合150、130和30 kDa抗原。我们得出结论，针对前4条抗原带的WBI对于最初使用市售猪肺炎支原体ELISA进行筛查的样本是一种有用的确认试验。
Zotero 插件