Interdisciplinary Graduate Program in Bioscience, Faculty of Science, Kasetsart University, 10900, Bangkok, Thailand.
Omics Center for Agriculture, Bioresources, Food, and Health, Kasetsart University (OmiKU), 10900, Bangkok, Thailand.
BMC Microbiol. 2022 Nov 11;22(1):272. doi: 10.1186/s12866-022-02683-y.
Pasteurella multocida is an opportunistic pathogen causing porcine respiratory diseases by co-infections with other bacterial and viral pathogens. Various bacterial genera isolated from porcine respiratory tracts were shown to inhibit the growth of the porcine isolates of P. multocida. However, molecular mechanisms during the interaction between P. multocida and these commensal bacteria had not been examined. METHODS: This study aimed to investigate the interaction between two porcine isolates of P. multocida (PM2 for type D and PM7 for type A) with Aeromonas caviae selected from the previously published work by co-culturing P. multocida in the conditioned media prepared from A. caviae growth and examining transcriptomic changes using RNA sequencing and bioinformatics analysis. RESULTS: In total, 629 differentially expressed genes were observed in the isolate with capsular type D, while 110 genes were significantly shown in type A. High expression of genes required for energy metabolisms, nutrient uptakes, and quorum sensing were keys to the growth and adaptation to the conditioned media, together with the decreased expression of those in the unurgent pathways, including translation and antibacterial resistance.
This transcriptomic analysis also displayed the distinct capability of the two isolates of P. multocida and the preference of the capsular type A isolate in response to the tough environment of the A. caviae conditioned media. Therefore, controlling the environmental sensing and nutrient acquisition mechanisms of P. multocida would possibly prevent the overpopulation of these bacteria and reduce the chance of becoming opportunistic pathogens.
多杀巴斯德氏菌是一种机会性病原体，通过与其他细菌和病毒病原体的共同感染引起猪的呼吸道疾病。从猪呼吸道中分离出的各种细菌属被证明可以抑制猪多杀巴斯德氏菌的生长。然而，在多杀巴斯德氏菌与这些共生细菌相互作用期间的分子机制尚未被研究。
本研究旨在通过共培养多杀巴斯德氏菌在从先前发表的工作中选择的气单胞菌生长的条件培养基中，并使用 RNA 测序和生物信息学分析检查转录组变化，来研究两种猪多杀巴斯德氏菌（PM2 为 D 型，PM7 为 A 型）与气单胞菌的相互作用。
在具有荚膜型 D 的分离株中观察到 629 个差异表达基因，而在 A 型中则有 110 个基因显著表达。能量代谢、营养摄取和群体感应所需基因的高表达是生长和适应条件培养基的关键，同时未紧急途径中的基因表达减少，包括翻译和抗菌抗性。
该转录组分析还显示了两种多杀巴斯德氏菌分离株的独特能力，以及荚膜型 A 分离株对气单胞菌条件培养基苛刻环境的偏好。因此，控制多杀巴斯德氏菌的环境感应和营养获取机制可能会阻止这些细菌的过度繁殖，并减少其成为机会性病原体的机会。
