Jantan Ibrahim, Raweh Salwa M, Yasin Yusyila Harlina Mohd, Murad Shahnaz
Department of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia.
Phytother Res. 2006 Jun;20(6):493-6. doi: 10.1002/ptr.1885.
Six aporphine and one phenanthrenoid alkaloids isolated from Aromadendron elegans Blume were investigated for their ability to inhibit arachidonic acid (AA), collagen and ADP induced platelet aggregation in human whole blood. The antiplatelet activity of the compounds was measured in vitro by the Chrono Log whole blood aggregometer using an electrical impedance method. Of the compounds tested, (-)-N-acetylnornuciferine, (-)-N-acetylanonaine and 1-(N-acetyl-N-methylamino)ethyl-3,4,6-trimethoxy-7-hydroxyphenanthrene showed strong inhibition on platelet aggregation caused by all three inducers. (-)-N-acetylanonaine was the most effective antiplatelet compound as it inhibited both arachidonic acid, collagen and ADP-induced platelet aggregation with IC(50) values of 66.1, 95.1 and 80.6 microm, respectively.
对从秀丽香树（Aromadendron elegans Blume）中分离得到的六种阿朴啡碱和一种菲类生物碱进行了研究，考察它们抑制花生四烯酸（AA）、胶原蛋白和ADP诱导人全血血小板聚集的能力。采用电阻抗法，通过Chrono Log全血凝集仪在体外测定这些化合物的抗血小板活性。在所测试的化合物中，（-）-N-乙酰去甲荷叶碱、（-）-N-乙酰阿那因和1-（N-乙酰-N-甲氨基）乙基-3,4,6-三甲氧基-7-羟基菲对由所有三种诱导剂引起的血小板聚集均表现出强烈抑制作用。（-）-N-乙酰阿那因是最有效的抗血小板化合物，因为它对花生四烯酸、胶原蛋白和ADP诱导的血小板聚集均有抑制作用，IC（50）值分别为66.1、95.1和80.6微摩尔。
