Chang F R, Wei J L, Teng C M, Wu Y C
Graduate Institute of Natural Products, Kaohsiung Medical College, Taiwan.
Phytochemistry. 1998 Dec;49(7):2015-8. doi: 10.1016/s0031-9422(98)00376-8.
Bioactivity-directed fractionation led to the isolation of two new 7-dehydroaporphine alkaloids, 7-hydroxy-dehydrothalicsimidine (1) and 7-formyl-dehydrothalicsimidine (2), along with the five known alkaloids, thalicsimidine (3), norpurpureine (4), N-methyllaurotetanine (5), lirinidine (6) and N-methylasimilobine (7), from the leaves of Annona purpurea. Structural elucidation of these compounds was established by mass and spectroscopic analyses. Among them, 1, 3, 4, 6 and 7 exhibited significant inhibition of collagen, arachidonic acid and platelet activating factor-induced platelet aggregation; 1 also showed inhibition against thrombin-induced platelet aggregation.
生物活性导向分离法从番荔枝叶中分离出两种新的7-脱氢阿朴啡生物碱，7-羟基-脱氢thalicsimidine（1）和7-甲酰基-脱氢thalicsimidine（2），以及五种已知生物碱，thalicsimidine（3）、去甲紫堇碱（4）、N-甲基劳罗替丁（5）、里里尼定（6）和N-甲基阿西米洛宾（7）。通过质谱和光谱分析确定了这些化合物的结构。其中，1、3、4、6和7对胶原、花生四烯酸和血小板活化因子诱导的血小板聚集具有显著抑制作用；1对凝血酶诱导的血小板聚集也有抑制作用。
