Lee Kang Dae, Lee Hwan Ho, Joo Ho Bum, Lee Hyoung Shin, Yu Tai Hyun, Chang Hee Kyung, Jeon Chang Ho, Park Jong Wook
Department of Otolaryngology, College of Medicine, Kosin University, Busan, Korea.
Anticancer Res. 2006 Mar-Apr;26(2B):1513-8.
The applicability of the melanoma antigen gene (MAGE) to the molecular diagnosis of cancer, by detecting MAGE mRNA in the sputa of head and neck cancer patients, was investigated.
Nested reverse transcription-polymerase chain reaction (RT-PCR) with the MAGE common primers designed to detect MAGE A1-6 genes was conducted with sputa of 17 head and neck squamous cell carcinoma patients.
Of the 17 sputa specimens from patients with squamous cell carcinoma, MAGE mRNA was detected in 13 cases, while the detection rate was 2 out of 40 in the normal control group, showing significant correlation (p<0.05) between head and neck squamous cell carcinoma and MAGE mRNA expression in sputa.
MAGE may be a useful tumor marker of head and neck cancer.
通过检测头颈癌患者痰液中的黑色素瘤抗原基因（MAGE）mRNA，研究MAGE在癌症分子诊断中的适用性。
对17名头颈鳞状细胞癌患者的痰液进行巢式逆转录聚合酶链反应（RT-PCR），使用设计用于检测MAGE A1-6基因的MAGE通用引物。
在17例鳞状细胞癌患者的痰液标本中，13例检测到MAGE mRNA，而正常对照组40例中有2例检测到，表明头颈鳞状细胞癌与痰液中MAGE mRNA表达之间存在显著相关性（p<0.05）。
MAGE可能是头颈癌的一种有用的肿瘤标志物。
