Puri Sarika N, Mendki M J, Sukumaran D, Ganesan K, Prakash Shri, Sekhar K
Defense Research and Development Establishment, Jhansi Road, Gwalior 474002, India.
J Med Entomol. 2006 Mar;43(2):207-13. doi: 10.1603/0022-2585(2006)043[0207:eabroc]2.0.co;2.
The southern house mosquito, Culex quinquefasciatus Say (Diptera: Culicidae), is closely associated with humans and is a vector of filariasis. Use of semiochemicals for control of mosquitoes is a novel and potentially ecofriendly control approach. Human skin emanations are known to attract mosquitoes. In the current study, olfactory and behavioral responses of host-seeking female Cx. quinquefasciatus to various chemical components of human skin emanations (carboxylic acids, alcohols, and aldehydes) were evaluated separately at three doses (0.01, 0.1, and 1 microg) for electroantennogram (EAG) and three doses (0.1, 1, and 10 microg) for behavioral assay. Results of EAG studies indicated that all carboxylic acids elicited significant olfactory responses except tetradecanoic acid (C14) and octadecanoic acid (C18). In particular, hexanoic acid (C6) elicited a maximum, eight-fold olfactory response compared with the solvent control. Ethylene glycol and benzyl alcohol exhibited significant EAG and behavioral responses, whereas among aldehydes, nonanal elicited high EAG and behavioral responses, but only at all three doses tested compared with control. Some carboxylic acids elicited significant behavioral responses, attracting approximately 75% of females tested toward chemical stimuli in a Y-tube olfactometer.
南方家蚊，致倦库蚊（双翅目：蚊科），与人类密切相关，是丝虫病的传播媒介。使用信息素控制蚊子是一种新颖且具有潜在生态友好性的控制方法。已知人类皮肤散发物会吸引蚊子。在当前研究中，分别以三种剂量（0.01、0.1和1微克）对寻找宿主的致倦库蚊雌蚊针对人类皮肤散发物的各种化学成分（羧酸、醇类和醛类）进行嗅觉和行为反应评估，用于触角电位（EAG）测试的为三种剂量（0.01、0.1和1微克），用于行为测定的为三种剂量（0.1、1和10微克）。EAG研究结果表明，除十四烷酸（C14）和十八烷酸（C18）外，所有羧酸均引发了显著的嗅觉反应。特别是，己酸（C6）引发的嗅觉反应最大，与溶剂对照相比高达八倍。乙二醇和苯甲醇表现出显著的EAG和行为反应，而在醛类中，壬醛引发了较高的EAG和行为反应，但仅在所有三种测试剂量下与对照相比才如此。一些羧酸引发了显著的行为反应，在Y型嗅觉仪中，约75%的受试雌蚊被化学刺激吸引。
