Bahk Song-Chul, Lee Sook-Hee, Jang Jung-Un, Choi Chang-Uk, Lee Bok-Soo, Chae Soo-Cheon, Song Hye-Jin, Park Zee-Yong, Yang Yun-Sik, Chung Hun-Taeg
Genome Research Center for Immune Disorders, School of Medicine, Wonkwang University, Iksan, Republic of Korea.
Proteomics. 2006 Jun;6(11):3436-44. doi: 10.1002/pmic.200500779.
Endotoxin-induced uveitis (EIU) is an animal model of acute ocular inflammation. To characterize the mechanism of EIU, we analyzed the infiltration of proteins in the vitreous bodies of rats with EIU and normal rats using 2-DE and micro LC/LC-MS/MS. Twenty spots were identified in vitreous bodies of rats. Eighteen of these spots were members of the crystallin family. The truncated form of beta A4- and beta B2-crystallin were predominant in normal vitreous bodies, but there were intact form of crystallins in lipopolysaccharide-injected rats with EIU. These results suggest that crystallin family proteins are the major group of proteins involved in uveitic vitreous and that C-terminal truncation of beta-crystallins may play a role in EIU-related disease progression.
内毒素诱导的葡萄膜炎（EIU）是一种急性眼部炎症的动物模型。为了阐明EIU的机制，我们使用二维电泳（2-DE）和微液相色谱/液相色谱-串联质谱（micro LC/LC-MS/MS）分析了EIU大鼠和正常大鼠玻璃体中蛋白质的浸润情况。在大鼠玻璃体中鉴定出20个斑点。其中18个斑点是晶状体蛋白家族的成员。βA4-和βB2-晶状体蛋白的截短形式在正常玻璃体中占主导地位，但在注射脂多糖的EIU大鼠中存在完整形式的晶状体蛋白。这些结果表明，晶状体蛋白家族蛋白是葡萄膜炎玻璃体中主要的蛋白质组，并且β-晶状体蛋白的C末端截短可能在EIU相关疾病进展中起作用。
