Cid J, Nogués N, Montero R, Hurtado M, Briega A, Parra R
Blood and Tissue Bank, Barcelona, Spain.
Transfus Med. 2006 Apr;16(2):131-6. doi: 10.1111/j.1365-3148.2006.00655.x.
The aims of the present study were to evaluate the estimated diagnostic accuracy of a new microtube column agglutination system (DG Gel, Diagnostic Grifols, Barcelona, Spain), to analyse the antibody reactivity and to compare the data with the two well-established DiaMed-ID and Ortho BioVue systems. We collected 3024 consecutive samples from blood donors, transfusion recipients and pregnant women, and 100 samples containing antibodies of known specificity. All these samples were tested in parallel by the three microtube agglutination systems. The estimated sensitivity was 100% for DG Gel and Ortho BioVue and 97.58% for DiaMed-ID. The estimated specificity was 99.93% for Ortho BioVue and 100% for DiaMed-ID and DG Gel. The score mean and range of the antibody titration of DG Gel, DiaMed-ID and Ortho BioVue were 34.31 (5-119), 30.3 (3-121) and 37.38 (3-112), respectively. All three column agglutination systems work well showing a high estimated diagnostic accuracy.
本研究的目的是评估一种新型微量管柱凝集系统（DG Gel，Diagnostic Grifols，巴塞罗那，西班牙）的估计诊断准确性，分析抗体反应性，并将数据与两种成熟的DiaMed-ID和Ortho BioVue系统进行比较。我们收集了来自献血者、输血受者和孕妇的3024份连续样本，以及100份含有已知特异性抗体的样本。所有这些样本均通过三种微量管凝集系统进行平行检测。DG Gel和Ortho BioVue的估计灵敏度为100%，DiaMed-ID为97.58%。Ortho BioVue的估计特异性为99.93%，DiaMed-ID和DG Gel为100%。DG Gel、DiaMed-ID和Ortho BioVue的抗体滴度评分均值及范围分别为34.31（5 - 119）、30.3（3 - 121）和37.38（3 - 112）。所有三种柱凝集系统表现良好，显示出较高的估计诊断准确性。
