Wang Chun-mei, Zheng Xue-li, Chen Xiao-guang
Department of Pathogenic Biology, Southern Medical University,Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2006 Apr;26(4):431-4.
To evaluate the toxicity of photoactivated insecticide K-01 to the larvae of Aedes albopictus and observe the histopathological changes in the larvae.
The number of dead larvae was recorded after application of K-01 at different concentrations under different illumination conditions. The content variation of the midgut, malpighian tubules and fat bodies in the larvae was observed microscopically, and the genomic DNA of the larvae was extracted for electrophoresis to identify the target bands.
The maximum larvae-killing effect was achieved with 50 mg/ml K-01 applied under sunlight (100% killing 24 hours after application). Optical microscopic observation of the killed larvae revealed severe damage of the mid-intestinal cells that showed disintegration and elongation. Distinct vacuoles were observed in the fat body cells, in which red droplets were seen to assemble around the cell nuclei. The result of 0.8% agarose gel electrophoresis of the larvae genomic DNA presented typical ladder patterns, suggesting the presence of cell apoptosis.
K-01 is an effective photoactivated insecticide.
评估光活化杀虫剂K-01对白纹伊蚊幼虫的毒性，并观察幼虫的组织病理学变化。
在不同光照条件下，应用不同浓度的K-01后记录死亡幼虫的数量。显微镜下观察幼虫中肠、马氏管和脂肪体的含量变化，并提取幼虫的基因组DNA进行电泳以鉴定目标条带。
在阳光下施用50 mg/ml K-01时，幼虫杀灭效果最佳（施用后24小时死亡率达100%）。光学显微镜观察死亡幼虫发现，中肠细胞严重受损，表现为解体和伸长。在脂肪体细胞中观察到明显的液泡，其中可见红色液滴围绕细胞核聚集。幼虫基因组DNA的0.8%琼脂糖凝胶电泳结果呈现典型的梯状条带，表明存在细胞凋亡。
K-01是一种有效的光活化杀虫剂。
