Chen Da-li, Huang Tao
School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2006 Apr;26(4):479-82.
To study the anti-tumor effect of Sanjie Kangliu (swelling-dispersing) Decoction in vivo and in vitro.
The mice bearing tumors (established by subcutaneous inoculation of SRS-82 cells) were fed with Sanjie Kangliu Decoction. The tumor was isolated and weighed to calculate the tumor inhibition rate, followed by morphological observation of the tumor cells under electron microscope. The DNA of the tumor cells was then extracted for agarose gel electrophoresis. In the in vitro experiment, cultured SRS-82 cells were treated with the serum of rats fed with Sanjie Kangliu Decoction, and the cell viability and apoptosis was examined by MTT assay and flow cytometry, respectively. Immunocytochemistry was employed to test the expression intensity of Bcl-2 protein of the rat serum-treated cells, and in situ hybridization performed to detect matrix metalloproteinase (MMP)-2 mRNA expression.
In the tumor-bearing mouse models, Sanjie Kangliu Decoction administration resulted in significant reduction of the tumor mass and apoptosis of the tumor cells as shown by typical apoptotic changes of the cell morphology and presence of DNA ladder in agarose gel electrophoresis. In comparison with the mice without the decoction treatment, the treated mice showed significantly lower tumor cell viability, higher cell apoptosis rate, and weaker Bcl-2 expression. The expression of MMP-2 mRNA showed no distinct difference between the groups.
Sanjie Kangliu Decoction has obvious anti-tumor effect both in vivo and in vitro possibly through the mechanism of inducing tumor cell apoptosis by reducing Bcl-2 expression without affecting the expression of MMP-2 mRNA.
研究散结抗瘤（消肿散结）汤的体内外抗肿瘤作用。
给接种SRS - 82细胞建立荷瘤模型的小鼠灌胃散结抗瘤汤。分离肿瘤并称重以计算肿瘤抑制率，随后在电子显微镜下对肿瘤细胞进行形态学观察。接着提取肿瘤细胞的DNA进行琼脂糖凝胶电泳。在体外实验中，用灌胃散结抗瘤汤的大鼠血清处理培养的SRS - 82细胞，分别通过MTT法和流式细胞术检测细胞活力和凋亡情况。采用免疫细胞化学法检测大鼠血清处理细胞的Bcl - 2蛋白表达强度，并用原位杂交法检测基质金属蛋白酶（MMP）-2 mRNA表达。
在荷瘤小鼠模型中，给予散结抗瘤汤后肿瘤质量显著减轻，肿瘤细胞出现凋亡，表现为细胞形态典型的凋亡变化以及琼脂糖凝胶电泳中出现DNA梯带。与未用该汤剂治疗的小鼠相比，治疗组小鼠的肿瘤细胞活力显著降低，细胞凋亡率更高，Bcl - 2表达较弱。两组之间MMP - 2 mRNA表达无明显差异。
散结抗瘤汤在体内外均有明显的抗肿瘤作用，其机制可能是通过降低Bcl - 2表达诱导肿瘤细胞凋亡，而不影响MMP - 2 mRNA的表达。
