Borisch B, Ellinger K, Neipel F, Fleckenstein B, Kirchner T, Ott M M, Müller-Hermelink H K
Department of Pathology, University of Würzburg, Federal Republic of Germany.
Virchows Arch B Cell Pathol Incl Mol Pathol. 1991;61(3):179-87. doi: 10.1007/BF02890420.
A newly described herpes virus, human herpes virus 6, (HHV-6), has been linked to exanthema subitum but beyond this its pathogenetic impact remains to be determined. A large body of evidence links it to various lymphoproliferative disorders and this study was conducted to identify forms of lymphoproliferation linked to HHV-6. We studied biopsy samples from 32 patients with disorders of the lymphatic system for the presence of HHV-6, both by polymerase chain reaction (PCR) and in-situ hybridization (ISH) methods, as well as Epstein-Barr virus (EBV) viral DNA, clonal rearrangements of the antigen receptor genes and bcl-2 genes. All the specimens were studied morphologically and a clinical follow-up of up to 4 years was obtained. Seven of the 32 patients were positive for HHV-6 DNA and the remainder were negative. Two of these HHV-6 positive specimens, both from elderly persons, showed a similar distinct histological pattern diagnosed as malignant B-cell lymphoma of high grade malignancy. Two other HHV-6-positive specimens were reactive lymphadenopathies occurring in younger adults. In addition, one further specimen with evidence of EBV-involvement was from a patient who died 3 months after biopsy with fatal infectious mononucleosis (IM). These five samples had HHV-6 DNA by PCR and ISH. Two specimens without specific histologic abnormalities showed evidence of HHV-6 only by PCR but not by ISH. Both high grade malignant lymphomas showed clonal proliferations, one of monoclonal B-cells and the other of clonal T-cells.(ABSTRACT TRUNCATED AT 250 WORDS)
一种新发现的疱疹病毒，即人类疱疹病毒6型（HHV-6），已被证实与幼儿急疹有关，但除此之外，其致病影响仍有待确定。大量证据表明它与多种淋巴增殖性疾病有关，本研究旨在确定与HHV-6相关的淋巴增殖形式。我们通过聚合酶链反应（PCR）和原位杂交（ISH）方法，研究了32例淋巴系统疾病患者的活检样本中HHV-6以及爱泼斯坦-巴尔病毒（EBV）的病毒DNA、抗原受体基因和bcl-2基因的克隆重排情况。对所有标本进行了形态学研究，并获得了长达4年的临床随访结果。32例患者中有7例HHV-6 DNA呈阳性，其余为阴性。这7例HHV-6阳性标本中有2例来自老年人，表现出相似的独特组织学模式，被诊断为高度恶性的B细胞淋巴瘤。另外2例HHV-6阳性标本是发生在年轻成年人中的反应性淋巴结病。此外，另1例有EBV感染证据的标本来自1例活检后3个月死于致命传染性单核细胞增多症（IM）的患者。这5个样本通过PCR和ISH检测均有HHV-6 DNA。2例无特定组织学异常的标本仅通过PCR检测出HHV-6，ISH检测为阴性。2例高度恶性淋巴瘤均显示克隆性增殖，1例为单克隆B细胞，另1例为克隆性T细胞。（摘要截选至250词）
