利用阵列辅助群体分离分析法在酿酒酵母中定位新性状
Mapping novel traits by array-assisted bulk segregant analysis in Saccharomyces cerevisiae.
作者信息
Brauer Matthew J, Christianson Cheryl M, Pai Dave A, Dunham Maitreya J
机构信息
Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA.
出版信息
Genetics. 2006 Jul;173(3):1813-6. doi: 10.1534/genetics.106.057927. Epub 2006 Apr 19.
Abstract
We demonstrate a new method, microarray-assisted bulk segregant analysis, for mapping traits in yeast by genotyping pooled segregants. We apply a probabilistic model to the progeny of a single cross and as little as two microarray hybridizations to reliably map an auxotrophic marker, a Mendelian trait, and a major-effect quantitative trait locus.
摘要
我们展示了一种新方法——微阵列辅助混合分离群体分析法，用于通过对混合分离群体进行基因分型来定位酵母中的性状。我们将概率模型应用于单个杂交的后代，并只需进行两次微阵列杂交，就能可靠地定位一个营养缺陷型标记、一个孟德尔性状和一个主效数量性状位点。
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