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利用阵列辅助群体分离分析法在酿酒酵母中定位新性状

Mapping novel traits by array-assisted bulk segregant analysis in Saccharomyces cerevisiae.

作者信息

Brauer Matthew J, Christianson Cheryl M, Pai Dave A, Dunham Maitreya J

机构信息

Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA.

出版信息

Genetics. 2006 Jul;173(3):1813-6. doi: 10.1534/genetics.106.057927. Epub 2006 Apr 19.

DOI:10.1534/genetics.106.057927
PMID:16624899
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1526703/
Abstract

We demonstrate a new method, microarray-assisted bulk segregant analysis, for mapping traits in yeast by genotyping pooled segregants. We apply a probabilistic model to the progeny of a single cross and as little as two microarray hybridizations to reliably map an auxotrophic marker, a Mendelian trait, and a major-effect quantitative trait locus.

摘要

我们展示了一种新方法——微阵列辅助混合分离群体分析法，用于通过对混合分离群体进行基因分型来定位酵母中的性状。我们将概率模型应用于单个杂交的后代，并只需进行两次微阵列杂交，就能可靠地定位一个营养缺陷型标记、一个孟德尔性状和一个主效数量性状位点。

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Isolation of Sets of a, α, a/α, a/a and α/α isogenic strains in Saccharomyces cerevisiae.在酿酒酵母中分离 a、α、a/α、a/a 和 α/α 同基因株系。
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Aep3p stabilizes the mitochondrial bicistronic mRNA encoding subunits 6 and 8 of the H+-translocating ATP synthase of Saccharomyces cerevisiae.Aep3p可稳定酿酒酵母H⁺转运ATP合酶亚基6和8的线粒体双顺反子mRNA。
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Genetic diversity in yeast assessed with whole-genome oligonucleotide arrays.利用全基因组寡核苷酸阵列评估酵母中的遗传多样性。
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