Zrimsek Petra, Kosec Marjan, Kunc Janez, Mrkun Janko
Clinic for Reproduction and Horses, Veterinary Faculty, University of Ljubljana, Gerbiceva 60, 1000 Ljubljana, Slovenia.
Asian J Androl. 2006 May;8(3):343-8. doi: 10.1111/j.1745-7262.2006.00130.x.
To assess that metabolic status of spermatozoa could provide a useful tool for evaluation of semen quality.
The accuracy of the spectrophotometric application of the resazurin reduction assay was assessed using receiver operating characteristic (ROC) analysis.
Areas under ROC curves (AUC) for motile sperm concentration and sperm index (SI) (sperm concentration multiplied by the square root of percentage sperm motility multiplied by the percentage normal sperm morphology) were 0.922. The best discrimination between poor and good semen samples according to the SI was achieved at a cut-off point of A610 = 0.209, where high sensitivity (94.1%) and specificity (91.7%) were calculated. The assay was less accurate when motile sperm concentration was used as the criterion value, yielding sensitivity of 88.2% and specificity of 87.5%, respectively. Likelihood ratios (LR) indicate that absorbances lower than 0.209 were at least 11.3 times as likely to be found in good semen samples than those in poor according to the SI, whereas in the case of motile sperm concentration, the LR was calculated to be 7.06.
These results show that the resazurin reduction assay combined with spectrophotometry is an accurate method of assessing the quality of boar semen.
评估精子的代谢状态能否为精液质量评估提供有用工具。
使用受试者工作特征（ROC）分析评估刃天青还原试验分光光度法应用的准确性。
活动精子浓度和精子指数（SI）（精子浓度乘以精子活力百分比的平方根乘以正常精子形态百分比）的ROC曲线下面积（AUC）为0.922。根据SI，在A610 = 0.209的截断点处，对不良和良好精液样本的区分最佳，计算得出高灵敏度（94.1%）和特异性（91.7%）。当将活动精子浓度用作标准值时，该试验准确性较低，灵敏度分别为88.2%，特异性为87.5%。似然比（LR）表明，根据SI，吸光度低于0.209在良好精液样本中出现的可能性至少是不良样本中的11.3倍，而对于活动精子浓度，LR计算为7.06。
这些结果表明，刃天青还原试验结合分光光度法是评估公猪精液质量的准确方法。
