Dart M G, Mesta J, Crenshaw C, Ericsson S A
Division of Range Animal Science, Sul Ross State University, Alpine, Texas 79832.
Arch Androl. 1994 Sep-Oct;33(2):71-5. doi: 10.3109/01485019408987806.
The objective of this study was to determine if reduction of the dye resazurin by bovine sperm is associated with the concentration of motile and progressively motile sperm. Metabolically active sperm reduce resazurin (blue color) to resorufin (pink color) and upon further reduction to dihydroresorufin (white color). A total of 68 semen samples from 20 Limousin bulls were collected using electroejaculation. The concentration of motile and progressively motile sperm was determined. Resazurin was added to each semen sample (8.8 microgram/mL semen), samples were then incubated at 37 degrees C for a maximum of 15 min, and visual color changes indicative of dye reduction were noted. Samples were identified as having either low fertility potential (< 200 x 10(6)/mL motile sperm and < 100 x 10(6)/mL progressively motile sperm) or high fertility potential (> or = 200 x 10(6)/mL motile sperm and > or = 100 x 10(6)/mL progressively motile sperm). Assessment of the reduction of resazurin from blue to pink (< or = 3.5 min) allowed identification of 88% of the low and 94% of the high fertility potential samples. In addition, the reduction of resazurin from blue to white (< 15 min) resulted in identification of 82% of the low and 76% of the high fertility potential samples. The modified resazurin reduction test is useful for determining the fertility potential of bovine spermatozoa.
本研究的目的是确定牛精子对刃天青的还原作用是否与活动精子和渐进性活动精子的浓度有关。具有代谢活性的精子将刃天青（蓝色）还原为试卤灵（粉红色），并进一步还原为二氢试卤灵（白色）。使用电刺激采精法从20头利木赞公牛身上采集了总共68份精液样本。测定了活动精子和渐进性活动精子的浓度。向每份精液样本中添加刃天青（8.8微克/毫升精液），然后将样本在37℃下孵育最多15分钟，并记录表明染料还原的视觉颜色变化。样本被鉴定为具有低生育潜力（活动精子<200×10⁶/毫升且渐进性活动精子<100×10⁶/毫升）或高生育潜力（活动精子≥200×10⁶/毫升且渐进性活动精子≥100×10⁶/毫升）。评估刃天青从蓝色还原为粉红色（≤3.5分钟）可识别出88%的低生育潜力样本和94%的高生育潜力样本。此外，刃天青从蓝色还原为白色（<15分钟）可识别出82%的低生育潜力样本和76%的高生育潜力样本。改良的刃天青还原试验有助于确定牛精子的生育潜力。
