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用刃天青还原试验评估精液质量。

Resazurin reduction test to evaluate semen quality.

作者信息

Reddy K V, Meherji P K, Gokral J S, Shahani S K

机构信息

Institute for Research in Reproduction (ICMR), Mumbai, India.

出版信息

Indian J Exp Biol. 1997 Apr;35(4):369-73.

PMID:9315237
Abstract

The resazurin test (REZ TEST) was performed on semen samples obtained from 75 untreated subfertile and 10 pregnancy confirmed fertile males. The results were compared with other sperm parameters to assess the quality of semen. The highest correlation of REZ grade was with motile sperm (r = 0.90, P < 0.001), sperm viability (r = 0.80, P < 0.001) and sperm concentration (r = 0.71, P < 0.001). The REZ grade indicated significant difference in the rate of positive tests between samples from fertile males and patients exhibiting abnormal semen parameters. The RES TEST had a positive predictive value of 93.75% for a progressives motile sperm concentration of > 20 x 106/ml and a negative predictive power of 88.7% for the same variable at a criterion value of 10 x 106/ml. It is suggested that REZ TEST can be performed with a relatively small volume of semen, it is easy to perform and requires no technical equipments.

摘要

对75名未经治疗的不育男性和10名经确认已怀孕的可育男性的精液样本进行了刃天青试验(REZ TEST)。将结果与其他精子参数进行比较,以评估精液质量。刃天青等级与活动精子的相关性最高(r = 0.90,P < 0.001)、精子活力(r = 0.80,P < 0.001)和精子浓度(r = 0.71,P < 0.001)。刃天青等级表明,可育男性样本与精液参数异常患者的样本之间,阳性检测率存在显著差异。对于进行性活动精子浓度>20×10⁶/ml,刃天青试验的阳性预测值为93.75%;对于同一变量,在临界值为10×10⁶/ml时,阴性预测能力为88.7%。建议刃天青试验可使用相对少量的精液进行,操作简便,无需技术设备。

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