Chu Kai On, Wang Chi Chiu, Chu Ching Yan, Chan Kwok Ping, Rogers Michael Scott, Choy Kwong Wai, Pang Chi Pui
Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, 3/F, Hong Kong Eye Hospital, 147K, Argyle Street, Kowloon, Hong Kong.
J Pharm Sci. 2006 Jun;95(6):1372-81. doi: 10.1002/jps.20594.
We carried out a pharmacokinetic study to determine the levels and profiles of catechins in pregnant rats and their fetuses after ingestion of green tea extract (GTE). We measured total catechin levels after enzyme digestions. Dams, at 15.5 days of gestation, were fed with GTE and catechins were measured in the maternal plasma, placenta, and fetus 0, 0.5, 1, 2, 3, 5, 8, 10, 12, 16, and 20 h after maternal GTE intake. The pharmacokinetic changes were analyzed by non compartmental models. We found that maternal plasma concentrations of catechins were about 10 times higher than in placenta and 50-100 times higher than in the fetus. AUC and Cmax levels of (-)-epicatechin (EC) were the highest in plasma while the levels of (-)-epigallocatechin gallate (EGCG) were the highest in the placenta and the fetus. The exposure level of catechin derivatives was higher than the gallate derivatives in maternal plasma after normalization but reversed in the placenta and fetus. The absorption of epi-isomers in plasma was found to be more favorable than their non epi-isomer counterparts. EGCG had the highest level of exposure (AUC) and the highest Cmax in the fetus, implying it may have potential for in utero antioxidant protection.
我们开展了一项药代动力学研究，以确定怀孕大鼠及其胎儿在摄入绿茶提取物（GTE）后儿茶素的水平和分布情况。我们在酶消化后测量了总儿茶素水平。在妊娠第15.5天，给孕鼠喂食GTE，并在母体摄入GTE后0、0.5、1、2、3、5、8、10、12、16和20小时测量母体血浆、胎盘和胎儿中的儿茶素。通过非房室模型分析药代动力学变化。我们发现，母体血浆中儿茶素的浓度比胎盘中的高约10倍，比胎儿中的高50 - 100倍。（-）-表儿茶素（EC）的AUC和Cmax水平在血浆中最高，而（-）-表没食子儿茶素没食子酸酯（EGCG）的水平在胎盘和胎儿中最高。标准化后，母体血浆中儿茶素衍生物的暴露水平高于没食子酸酯衍生物，但在胎盘和胎儿中则相反。发现血浆中表异构儿茶素的吸收比其非表异构对应物更有利。EGCG在胎儿中的暴露水平（AUC）最高，Cmax也最高，这意味着它可能具有子宫内抗氧化保护的潜力。
