Dang Yongyan, Ren Qiushi, Li Wanrong, Yang Qing, Zhang Jinsheng
Institute for Laser Medicine & Biophotonics, Shanghai Jiaotong University, Shanghai, China.
Skin Res Technol. 2006 May;12(2):119-25. doi: 10.1111/j.0909-752X.2006.00137.x.
BACKGROUNDS/AIMS: The aim of the study was to compare the changes of the biophysical properties and to objectify the effects of 595 nm pulsed dye, 1064 nm Q-switched Nd:YAG and 1320 nm Nd:YAG lasers non-ablative rejuvenation by non-invasive techniques.
KM mice were used for the study. The 595 nm pulsed dye, 1064 nm Q-switched Nd:YAG and 1320 nm Nd:YAG laser treatments were evaluated with biophysical parameter measurements including skin elasticity, skin color, skin trans-epidermal water loss (TEWL) and skin hydration.
All three lasers improved the biophysical properties in the skin of KM mice. In skin elasticity measurements, the 1064 nm laser treatment showed the lowest ratio (0.61+/-0.09) while the 1320 nm laser showed the highest one (0.76+/-0.07) on day 60. For erythema values, a significant increase was observed immediately after the 1064 nm laser treatment (196.67+/-19.17), but the lowest values occurred with the 1320 nm laser treatment (189.83+/-16.54). None of the three lasers resulted in obvious changes of skin melanin. TEWL increased immediately after laser irradiation, then began to recover and decreased 60 days after the 595 and 1064 nm laser treatments. With the 1320 nm laser treatment the TEWL began to decrease from day 7 and obtained the lowest mean values (5.23+/-1.13). The water-holding capacity increased initially for the 595 and 1320 nm laser irradiation, while decreased for the 1064 nm laser. At day 60 of the experiment, skin hydration values in all animals were superior to those of the controls. The 1320 nm laser treatment caused the highest ratio (1.29+/-0.26). Both the values of TEWL and skin hydration for the 1320 nm laser treated areas differed significantly from the other two lasers.
Our data showed the 1064 nm Q-switched Nd:YAG laser treatment was most effective in improving the skins' mechanical properties, while the 1320 nm Nd:YAG laser can enhance greatly the skin barrier function and the water-holding capacity. Moreover, we demonstrated the biophysical properties differed considerably between different areas.
背景/目的：本研究旨在比较生物物理特性的变化，并通过非侵入性技术客观评估595nm脉冲染料激光、1064nm调Q Nd:YAG激光和1320nm Nd:YAG激光非剥脱性嫩肤的效果。
采用KM小鼠进行研究。通过测量包括皮肤弹性、皮肤颜色、经表皮水分流失（TEWL）和皮肤水合作用等生物物理参数，对595nm脉冲染料激光、1064nm调Q Nd:YAG激光和1320nm Nd:YAG激光治疗进行评估。
所有三种激光均改善了KM小鼠皮肤的生物物理特性。在皮肤弹性测量中，第60天时1064nm激光治疗组的比值最低（0.61±0.09），而1320nm激光治疗组的比值最高（0.76±0.07）。对于红斑值，1064nm激光治疗后立即观察到显著增加（196.67±19.17），但1320nm激光治疗组的值最低（189.83±16.54）。三种激光均未导致皮肤黑色素明显变化。激光照射后TEWL立即增加，然后开始恢复，595nm和1064nm激光治疗60天后TEWL降低。1320nm激光治疗后，TEWL从第7天开始下降，并获得最低平均值（5.23±1.13）。595nm和1320nm激光照射最初使皮肤持水能力增加，而1064nm激光使其降低。实验第60天时，所有动物的皮肤水合值均优于对照组。1320nm激光治疗组的比值最高（1.29±0.26）。1320nm激光治疗区域的TEWL值和皮肤水合值与其他两种激光有显著差异。
我们的数据表明，1064nm调Q Nd:YAG激光治疗在改善皮肤机械性能方面最有效，而1320nm Nd:YAG激光可显著增强皮肤屏障功能和持水能力。此外，我们证明不同区域的生物物理特性存在显著差异。
