Kim T J, Cho H S, Park N Y, Lee J I
School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, WA 6150, Australia.
J Vet Med B Infect Dis Vet Public Health. 2006 Mar;53(2):87-90. doi: 10.1111/j.1439-0450.2006.00917.x.
A protein chip based on surface plasmon resonance (SPR) was developed for measuring the Mycoplasma hyopneumoniae antibody titres using a recombinant 30-kDa fragment of P97 adhesin as an antigen. The diagnostic potential of this SPR assay, for detecting the antibody titres to the M. hyopneumoniae 30-kDa protein, was compared with that of conventional ELISA using 70 pig serum samples taken from six pig farms. The SPR assay was found to be highly specific and sensitive. Moreover, there was a strong positive correlation between the SPR and ELISA titres (n = 70, r = 0.898, P < 0.01). Therefore, this recombinant 30-kDa protein can be used as an antigen for serological studies, and the SPR, which is a label-free method, is expected to be a valuable and reproducible tool in the serodiagnosis of M. hyopneumoniae infection.
开发了一种基于表面等离子体共振（SPR）的蛋白质芯片，用于以重组P97黏附素30 kDa片段作为抗原检测猪肺炎支原体抗体滴度。使用从六个养猪场采集的70份猪血清样本，将这种SPR检测法检测猪肺炎支原体30 kDa蛋白抗体滴度的诊断潜力与传统酶联免疫吸附测定（ELISA）进行了比较。结果发现SPR检测法具有高度特异性和敏感性。此外，SPR和ELISA滴度之间存在强正相关（n = 70，r = 0.898，P < 0.01）。因此，这种重组30 kDa蛋白可作为血清学研究的抗原，而SPR作为一种无标记方法，有望成为猪肺炎支原体感染血清诊断中有价值且可重复的工具。
