Cho Ho-Seong, Park Nam-Yong
Department of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University, Gwangju, Korea.
J Vet Med Sci. 2006 Dec;68(12):1327-9. doi: 10.1292/jvms.68.1327.
A protein chip based on surface plasmon resonance (SPR) was developed to measure the antibody (Ab) titers of classical swine fever virus (CSFV) using the recombinant gp55 protein as an antigen. The diagnostic potential of this SPR assay for detecting the Ab titers to CSFV gp55 was compared that of the enzyme-linked immunosorbent assay (ELISA) using 170 serum samples from 14 pig farms. The SPR assay was highly specific and sensitive, and there were no cross-reactions detected. There was a strong positive correlation between the SPR and ELISA titers (n=170, r=0.869, p<0.01). Therefore, the SPR label-free method is a valuable tool in the serodiagnosis of CSFV infection and determining Ab titers after vaccination.
开发了一种基于表面等离子体共振（SPR）的蛋白质芯片，以重组gp55蛋白为抗原，用于检测经典猪瘟病毒（CSFV）的抗体（Ab）效价。使用来自14个猪场的170份血清样本，比较了这种SPR检测法检测CSFV gp55抗体效价的诊断潜力与酶联免疫吸附测定（ELISA）的诊断潜力。SPR检测法具有高度特异性和敏感性，未检测到交叉反应。SPR和ELISA效价之间存在强正相关（n = 170，r = 0.869，p <0.01）。因此，SPR无标记方法是CSFV感染血清学诊断和疫苗接种后抗体效价测定的有价值工具。
