May M, Helbl V, Pfister H, Fuchs P G
Institut für Klinische und Molekulare Virologie, Friedrich-Alexander Universität Erlangen-Nürnberg, Germany.
J Gen Virol. 1991 Dec;72 ( Pt 12):2989-97. doi: 10.1099/0022-1317-72-12-2989.
The non-coding region (NCR) of the epidermodysplasia verruciformis (EV)-associated human papillomavirus 8 (HPV-8) has been investigated for sequence-specific DNA-protein interactions with nuclear proteins from epithelial HeLa cells. Using DNase I protection analysis 18 footprints could be found within the HPV-8 NCR, covering altogether over 60% of both DNA strands. Several footprints coincided with the known binding sites of transcription factors (NF1, AP1, octamer and PEA3 consensus sequences); the other displayed no obvious similarities in this regard. The overall distribution of sequences involved in DNA-protein interactions differed clearly from the binding patterns reported for other HPVs. Parts of the two binding sites for the viral trans-activator protein E2 were shown to interact with non-E2 factors. The EV-specific NCR motifs M33, M29 and A/T box were all involved in protein binding. By comparing the footprints within the respective motifs of the closely related types HPV-8 and -19, quantitative and qualitative differences were detected for M33 and the A/T box.
对疣状表皮发育不良（EV）相关的人乳头瘤病毒8型（HPV - 8）的非编码区（NCR）进行了研究，以探讨其与上皮性HeLa细胞核蛋白的序列特异性DNA - 蛋白质相互作用。使用DNase I保护分析，在HPV - 8 NCR内可发现18个足迹，共覆盖两条DNA链的60%以上。几个足迹与转录因子（NF1、AP1、八聚体和PEA3共有序列）的已知结合位点重合；其他足迹在这方面没有明显的相似性。参与DNA - 蛋白质相互作用的序列的总体分布与其他HPV报道的结合模式明显不同。病毒反式激活蛋白E2的两个结合位点的部分区域显示与非E2因子相互作用。EV特异性NCR基序M33、M29和A/T盒均参与蛋白质结合。通过比较密切相关的HPV - 8和 - 19型各自基序内的足迹，检测到M33和A/T盒在数量和质量上的差异。
