Kiyono T, Hiraiwa A, Ishibashi M
Laboratory of Viral Oncology, Aichi Cancer Center, Nagoya, Japan.
Virology. 1992 Feb;186(2):628-39. doi: 10.1016/0042-6822(92)90029-o.
Using the polymerase chain reaction technique, we cloned and sequenced DNA fragments containing the E6 genes of the epidermodysplasia verruciformis (EV)-associated HPVs 5, 8, 14, 20, 21, 25, and 47, of which only the sequences of HPVs 5, 8, and 47 have previously been reported. Based on the deduced amino acid sequence homology (57.3 to 83.0%), these HPVs could be divided into two clusters: HPVs 5, 8, and 47, and HPVs 14, 20, 21, and 25. The E6 genes of three HPVs from each cluster were examined for transforming activity toward a cultured rat fibroblast cell line, 3Y1, using the retrovirus-mediated gene transfer technique, and all were found to induce morphological transformation. However, the E6 genes of the first cluster were more potent than those of the second. Since HPVs 5 and 8 are the most frequently found HPVs in malignant lesions of EV patients, the observed in vitro transforming activities of the E6 genes may reflect their oncogenic potential in humans.
我们运用聚合酶链反应技术，克隆并测序了包含疣状表皮发育不良（EV）相关人乳头瘤病毒（HPV）5、8、14、20、21、25和47的E6基因的DNA片段，其中仅有HPV 5、8和47的序列先前有过报道。基于推导的氨基酸序列同源性（57.3%至83.0%），这些HPV可分为两个簇：HPV 5、8和47，以及HPV 14、20、21和25。利用逆转录病毒介导的基因转移技术，检测了每个簇中三种HPV的E6基因对培养的大鼠成纤维细胞系3Y1的转化活性，结果发现所有基因均能诱导形态转化。然而，第一簇的E6基因比第二簇的更具活性。由于HPV 5和8是EV患者恶性病变中最常见的HPV，所观察到的E6基因在体外的转化活性可能反映了它们在人类中的致癌潜力。
