Dlugosch D, Eis-Hübinger A M, Kleim J P, Kaiser R, Bierhoff E, Schneweis K E
Institute of Medical Microbiology and Immunology, University of Bonn, Germany.
J Med Virol. 1991 Oct;35(2):136-41. doi: 10.1002/jmv.1890350212.
A simplified assay for the diagnosis of varicella-zoster virus (VZV) infections based on the polymerase chain reaction (PCR) is described. Omitting the procedures for extraction and purification of DNA, the crude vesicle fluid materials were used for PCR. Moreover, hybridization was not necessary for detection of the amplification products because they were already visible after ethidium bromide staining of the electrophoresis gel. Results could therefore be obtained within one day. In comparison to virus isolation, PCR proved much more rapid, highly sensitive, and specific. DNA extraction and a double PCR assay with nested primers were necessary for detection of latent VZV infections in trigeminal and thoracic ganglia. The data suggest that the procedures described are universally applicable to several types of specimens dependent on the calculated amount of target DNA.
本文描述了一种基于聚合酶链反应（PCR）的用于诊断水痘带状疱疹病毒（VZV）感染的简化检测方法。省去DNA提取和纯化步骤，将粗制的水疱液材料用于PCR。此外，检测扩增产物无需杂交，因为在电泳凝胶用溴化乙锭染色后扩增产物已清晰可见。因此可在一天内获得结果。与病毒分离相比，PCR方法被证明更快、灵敏度更高且特异性更强。检测三叉神经节和胸神经节中的潜伏性VZV感染需要进行DNA提取和使用巢式引物的双重PCR检测。数据表明，所描述的方法普遍适用于根据目标DNA计算量而定的几种类型的标本。
