Auman Ann J, Breezee Jennifer L, Gosink John J, Kämpfer Peter, Staley James T
Department of Biology, Pacific Lutheran University, Tacoma, WA 98447, USA.
Department of Microbiology, University of Washington, Seattle, WA 98195, USA.
Int J Syst Evol Microbiol. 2006 May;56(Pt 5):1001-1007. doi: 10.1099/ijs.0.64068-0.
A gas vacuolate bacterium, designated strain 37T, was isolated from a sea ice core collected from Point Barrow, Alaska, USA. Cells of strain 37T were large (6-14 microm in length), rod-shaped, contained gas vacuoles of two distinct morphologies, and grew well at NaCl concentrations of 1-10 % and at temperatures of -12 to 10 degrees C. The DNA G+C content was 40 mol%. Whole-cell fatty acid analysis showed that 16 : 1omega7c comprised 67 % of the total fatty acid content. Phylogenetic analysis of 16S rRNA gene sequences indicated that this bacterium was closely related to members of the genus Psychromonas, with highest sequence similarity (96.8 %) to Psychromonas antarctica. Phenotypic analysis differentiated strain 37T from P. antarctica on the basis of several characteristics, including cell morphology, growth temperature range and the ability to hydrolyse polymers. DNA-DNA hybridization experiments revealed a level of relatedness of 37 % between strain 37T and P. antarctica, providing further support that it represents a distinct species. The name Psychromonas ingrahamii sp. nov. is proposed for this novel species. The type strain is 37T (=CCUG 51855T=CIP 108865T).
从美国阿拉斯加巴罗角采集的海冰芯中分离出一株产气泡的细菌，命名为37T菌株。37T菌株的细胞较大（长度为6 - 14微米），呈杆状，含有两种不同形态的气泡，在1% - 10%的NaCl浓度和 - 12至10摄氏度的温度下生长良好。DNA的G + C含量为40摩尔%。全细胞脂肪酸分析表明，16 : 1ω7c占总脂肪酸含量的67%。16S rRNA基因序列的系统发育分析表明，该细菌与嗜冷单胞菌属的成员密切相关，与南极嗜冷单胞菌的序列相似性最高（96.8%）。表型分析基于包括细胞形态、生长温度范围和水解聚合物能力等几个特征，将37T菌株与南极嗜冷单胞菌区分开来。DNA - DNA杂交实验表明，37T菌株与南极嗜冷单胞菌之间的相关性水平为37%，进一步支持它代表一个独特的物种。为此新物种提议命名为英格拉姆嗜冷单胞菌（Psychromonas ingrahamii sp. nov.）。模式菌株为37T（=CCUG 51855T = CIP 108865T）。
