College of Chemical and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266510, PR China.
The State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, PR China.
Int J Syst Evol Microbiol. 2011 Sep;61(Pt 9):2052-2057. doi: 10.1099/ijs.0.026153-0. Epub 2010 Sep 17.
Two Gram-negative, motile, aerobic, catalase- and oxidase-positive, rod-shaped strains, designated ZS5-23(T) and ZS6-22(T), were respectively isolated from Antarctic coastal attached (fast) ice and surface seawater samples. Both strains could grow at 4-35 °C (optimum 30 °C) and in the absence of NaCl. Analyses of 16S rRNA gene sequences revealed that strains ZS5-23(T) and ZS6-22(T) were closely related to each other (99.0 % sequence similarity) and belonged to the class Gammaproteobacteria, with their closest relatives being Spongiibacter and Melitea species (93.1-94.3 % sequence similarity). The predominant cellular fatty acids in both strains were C₁₇:₁ω8c, C₁₇:₀ and summed feature 3 (C₁₆:₁ω7c and/or iso-C₁₅:₀ 2-OH). Genomic DNA G+C contents of strains ZS5-23(T) and ZS6-22(T) were 51.5 and 51.8 mol%, respectively. The DNA-DNA relatedness between strains ZS5-23(T) and ZS6-22(T) was 50.9 %. Strains ZS5-23(T) and ZS6-22(T) could be differentiated from each other and from Spongiibacter and Melitea species by differences in a number of phenotypic properties. Based on the data presented, strains ZS5-23(T) and ZS6-22(T) represent two novel species in a new genus in the class Gammaproteobacteria, for which the names Zhongshania antarctica gen. nov., sp. nov. (the type species) and Zhongshania guokunii sp. nov. are proposed. The type strain of Zhongshania antarctica is ZS5-23(T) ( = KACC 14066(T) = CCTCC AB 209246(T)) and that of Zhongshania guokunii is ZS6-22(T) ( = KACC 14532(T) = CCTCC AB 209247(T)).
两株革兰氏阴性、运动性、需氧、过氧化氢酶和氧化酶阳性、杆状的菌株，分别命名为 ZS5-23(T) 和 ZS6-22(T)，分别从南极沿海附着（快速）冰和表层海水样本中分离得到。这两种菌株都可以在 4-35°C（最佳 30°C）和无 NaCl 的条件下生长。16S rRNA 基因序列分析表明，菌株 ZS5-23(T) 和 ZS6-22(T) 彼此之间密切相关（序列相似度为 99.0%），属于γ变形菌纲，与其最接近的是海绵菌属和梅利特菌属（序列相似度为 93.1-94.3%）。两种菌株的主要细胞脂肪酸为 C₁₇:₁ω8c、C₁₇:₀ 和总和特征 3（C₁₆:₁ω7c 和/或 iso-C₁₅:₀ 2-OH）。菌株 ZS5-23(T) 和 ZS6-22(T) 的基因组 DNA G+C 含量分别为 51.5 和 51.8 mol%。菌株 ZS5-23(T) 和 ZS6-22(T) 之间的 DNA-DNA 相关性为 50.9%。菌株 ZS5-23(T) 和 ZS6-22(T) 可以通过一些表型特性的差异与彼此以及海绵菌属和梅利特菌属区分开来。基于所提供的资料，菌株 ZS5-23(T) 和 ZS6-22(T) 代表γ变形菌纲一个新属中的两个新种，分别命名为南极洲中菌属（Zhongshania）nov. 属，南极中菌种（Zhongshania antarctica）sp. nov.（模式种）和中国古菌种（Zhongshania guokunii）sp. nov.。南极洲中菌的模式菌株为 ZS5-23(T)（=KACC 14066(T)=CCTCC AB 209246(T)），中国古菌的模式菌株为 ZS6-22(T)（=KACC 14532(T)=CCTCC AB 209247(T)）。
