Kwon Kae Kyoung, Lee Soon Jae, Park Jae Hyun, Ahn Tae-Young, Lee Hong Kum
Marine Biotechnology Research Center, Korea Ocean Research and Development Institute (KORDI), Ansan PO Box 29, 425-600, Republic of Korea.
Department of Microbiology, Dankook University, Cheonan 330-714, Republic of Korea.
Int J Syst Evol Microbiol. 2006 May;56(Pt 5):1055-1058. doi: 10.1099/ijs.0.64171-0.
A number of marine bacteria isolated from young biofilms were characterized as belonging to the family Flavobacteriaceae. The taxonomic characterization of strain KOPRI 13649T, which was isolated from an acrylic surface at the seashore at Gangneung, Korea, is reported here. The nearly complete 16S rRNA gene sequence of strain KOPRI 13649T was determined and was found to have a high level of similarity with that of Psychroserpens burtonensis (95.0-95.6 %). In addition, phylogenetic analysis and comparison with closely related strains confirmed that the strain represented a novel member of the genus Psychroserpens. The major respiratory quinone of strain KOPRI 13649T was MK-6 and the DNA G+C content was 29.8 mol%. The dominant fatty acid methyl esters were i-15 : 0, a-15 : 0, i-16 : 0, i-15 : 1omega10, 16 : 1omega7 and 15 : 0. Growth was observed at 10-34 degrees C (optimum 30 degrees C), at pH 6-9 (optimum 6.5-8.0) and with 0.5-4 % NaCl (optimum 1 %). On the basis of the polyphasic taxonomic evidence presented, strain KOPRI 13649T (=KCCM 42261T=JCM 13413T) should be classified as the type strain of a novel species in the genus Psychroserpens, for which the name Psychroserpens mesophilus sp. nov. is proposed.
从年轻生物膜中分离出的许多海洋细菌被鉴定为黄杆菌科。本文报道了从韩国江陵海边丙烯酸表面分离出的菌株KOPRI 13649T的分类学特征。测定了菌株KOPRI 13649T几乎完整的16S rRNA基因序列，发现其与伯顿嗜冷蛇菌（Psychroserpens burtonensis）的序列具有高度相似性（95.0 - 95.6%）。此外，系统发育分析以及与密切相关菌株的比较证实，该菌株代表了嗜冷蛇菌属的一个新成员。菌株KOPRI 13649T的主要呼吸醌为MK - 6，DNA G + C含量为29.8 mol%。主要的脂肪酸甲酯为i - 15 : 0、a - 15 : 0、i - 16 : 0、i - 15 : 1ω10、16 : 1ω7和15 : 0。在10 - 34℃（最适温度30℃）、pH 6 - 9（最适pH 6.5 - 8.0）以及0.5 - 4% NaCl（最适1%）条件下可观察到生长。基于所提供的多相分类学证据，菌株KOPRI 13649T（=KCCM 42261T = JCM 13413T）应被归类为嗜冷蛇菌属一个新物种的模式菌株，为此提议将其命名为嗜温嗜冷蛇菌（Psychroserpens mesophilus sp. nov.）。
