Department of Chemical Engineering, Pohang University of Science and Technology, San 31, Hyoja-Dong, Pohang 790-784, Republic of Korea.
Gyeongbuk Sea Grant Institute, Pohang University of Science and Technology, San 31, Hyoja-Dong, Pohang 790-784, Republic of Korea.
Int J Syst Evol Microbiol. 2013 Feb;63(Pt 2):703-708. doi: 10.1099/ijs.0.041632-0. Epub 2012 Apr 27.
A novel bacterium, designated strain F051-1(T), isolated from a seawater sample collected from the coast at Damupo beach in Pohang, Korea, was investigated in a polyphasic taxonomic study. Cells were yellow-pigmented, strictly aerobic, Gram-staining-negative and rod-shaped. The temperature, pH and NaCl ranges for growth were 4-30 °C, pH 6.0-9.0 and 1.0-6.0 % (w/v), respectively. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain F051-1(T) belongs to the genus Psychroserpens in the family Flavobacteriaceae. Its closest relatives were Psychroserpens burtonensis ACAM 188(T) (96.8 % 16S rRNA gene sequence similarity) and Psychroserpens mesophilus KOPRI 13649(T) (95.7 %). The major cellular fatty acids were iso-C(15 : 0), iso-C(15 : 1) G and anteiso-C(15 : 0). The polar lipid profile consisted of a mixture of phosphatidylethanolamine, two unidentified aminolipids, one unidentified phospholipid and eight unidentified lipids. The major respiratory quinone was menaquinone-6 and the genomic DNA G+C content of the strain was 33.5 mol%. On the basis of phenotypic, phylogenetic and genotypic data, strain F051-1(T) represents a novel species within the genus Psychroserpens, for which the name Psychroserpens damuponensis sp. nov. is proposed. The type strain is F051-1(T) ( = KCTC 23539(T) = JCM 17632(T)).
一株从韩国浦项市 Damupo 海滩海水样本中分离得到的海洋细菌 F051-1(T)，经多相分类学研究后进行了鉴定。该菌细胞呈黄色，严格需氧，革兰氏染色阴性，呈杆状。最适生长温度、pH 值和盐度范围分别为 4-30°C、pH6.0-9.0 和 1.0-6.0%（w/v）。16S rRNA 基因序列系统发育分析表明，菌株 F051-1(T) 属于黄杆菌科 Psychroserpens 属。其最接近的亲缘种是 Psychroserpens burtonensis ACAM 188(T)（96.8% 16S rRNA 基因序列相似性）和 Psychroserpens mesophilus KOPRI 13649(T)（95.7% 16S rRNA 基因序列相似性）。主要细胞脂肪酸为 iso-C(15:0)、iso-C(15:1) G 和 anteiso-C(15:0)。极性脂谱由磷脂酰乙醇胺、两种未鉴定的胺脂、一种未鉴定的磷脂和八种未鉴定的脂类组成。主要呼吸醌为menaquinone-6，基因组 DNA G+C 含量为 33.5 mol%。基于表型、系统发育和基因型数据，菌株 F051-1(T) 代表 Psychroserpens 属中的一个新种，建议将其命名为 Psychroserpens damuponensis sp. nov.。模式菌株为 F051-1(T)（=KCTC 23539(T)=JCM 17632(T)）。
