Ho H M, Ohshima Ken-Ichi, Watanabe Gen, Taya Kazuyoshi, Strawn E Y, Hutz Reinhold J
Medical College of Wisconsin, USA.
J Reprod Dev. 2006 Aug;52(4):523-8. doi: 10.1262/jrd.18006. Epub 2006 Apr 21.
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is the most toxic of the halogenated dioxins and one of the most poisonous substances known to man. The major toxic effects of TCDD on reproduction are decreased fertility and diminished ability to maintain a pregnancy. Granulosa cells obtained from hormonally stimulated women participating in an in-vitro fertilization program were cultured with 3.1 femtomolar, 3.1 picomolar and 3.1 nanomolar TCDD. While inhibin B production was not altered, inhibin A production increased significantly after 4 hours of exposure to both nanomolar and micromolar TCDD concentrations. By 8 hours of exposure to these concentrations of dioxin, human luteinizing granulosa cells exhibited a pronounced increase in inhibin A, nearly quadrupling secretion from unexposed control cells. TCDD continued to increase inhibin A secretion at the picomolar concentration at 24 and 36 hours. It is conceivable that TCDD may act at the ovary to augment inhibin A secretion, thereby reducing FSH-stimulable estrogen secretion by granulosa cells.
2,3,7,8-四氯二苯并-对-二恶英（TCDD）是卤代二恶英中毒性最强的，也是人类已知的最有毒物质之一。TCDD对生殖的主要毒性作用是生育能力下降和维持妊娠的能力减弱。从参与体外受精项目且接受激素刺激的女性身上获取的颗粒细胞，分别用3.1飞摩尔、3.1皮摩尔和3.1纳摩尔的TCDD进行培养。虽然抑制素B的产生没有改变，但在暴露于纳摩尔和微摩尔浓度的TCDD 4小时后，抑制素A的产生显著增加。在暴露于这些二恶英浓度8小时后，人黄体化颗粒细胞的抑制素A显著增加，分泌量几乎是未暴露对照细胞的四倍。在24小时和36小时时，皮摩尔浓度的TCDD持续增加抑制素A的分泌。可以想象，TCDD可能作用于卵巢以增加抑制素A的分泌，从而减少颗粒细胞对促卵泡生成素刺激的雌激素分泌。
