Goodman Catherine M, Chari Nandini S, Han Gang, Hong Rui, Ghosh Partha, Rotello Vincent M
Department of Chemistry, University of Massachusetts, Amherst, MA 01003, USA.
Chem Biol Drug Des. 2006 Apr;67(4):297-304. doi: 10.1111/j.1747-0285.2006.00372.x.
A family of nanoparticles featuring surfaces of varying hydrophobicity was synthesized. The efficiency of DNA-binding was determined, demonstrating in a fivefold modulation in binding a 37-mer DNA strand. Nanoparticle-binding causes a reversible conformational change in the DNA structure, as demonstrated by circular dichroism and fluorescence experiments. Furthermore, the affinity of the nanoparticle for the DNA can be regulated by external agents, though stability of the complex is observed at relatively high ionic strengths.
合成了一族具有不同疏水性表面的纳米颗粒。测定了DNA结合效率，结果表明在结合一条37聚体DNA链时存在5倍的调节作用。如圆二色性和荧光实验所示，纳米颗粒结合会导致DNA结构发生可逆的构象变化。此外，纳米颗粒对DNA的亲和力可由外部试剂调节，不过在相对较高的离子强度下观察到复合物具有稳定性。
