Li Peicai, Zhang Lixue, Ai Kelong, Li Dan, Liu Xiaohua, Wang Erkang
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun, Jilin 130022, PR China.
J Control Release. 2008 Jul 14;129(2):128-34. doi: 10.1016/j.jconrel.2008.04.006. Epub 2008 Apr 18.
The stability of the complex of cationic lipid with nucleic acid, especially when facing serum, is crucial for the efficiency of gene delivery. Here, we demonstrated that the stability of the complex of didodecyldimethylammonium bromide (DDAB, a cationic lipid) with DNA in the presence of serum dramatically increased after coating DDAB onto the surface of the gold nanoparticles. The stability of the complex was demonstrated with dye intercalation assay, and agarose gel electrophoresis. The process of the interaction was characterized with UV-vis spectra and the morphology of the complex was observed with atomic force microscope (AFM). Cell viability assays demonstrated that the cytotoxicity of DDAB was also decreased.
阳离子脂质与核酸复合物的稳定性，尤其是在面对血清时，对于基因递送效率至关重要。在此，我们证明，在将十二烷基二甲基溴化铵（DDAB，一种阳离子脂质）包被在金纳米颗粒表面后，其与DNA在血清存在下的复合物稳定性显著提高。通过染料嵌入测定法和琼脂糖凝胶电泳证明了复合物的稳定性。用紫外可见光谱表征了相互作用过程，并用原子力显微镜（AFM）观察了复合物的形态。细胞活力测定表明，DDAB的细胞毒性也降低了。
