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用金纳米颗粒修饰单个巨型DNA。

Decorating a single giant DNA with gold nanoparticles.

作者信息

Carnerero Jose M, Masuoka Shinsuke, Baba Hikari, Yoshikawa Yuko, Prado-Gotor Rafael, Yoshikawa Kenichi

机构信息

Department of Physical Chemistry, Faculty of Chemistry, Universidad de Sevilla c/Profesor García González, 1 41012 Seville Spain

Faculty of Life and Medical Sciences, Doshisha University Kyotanabe 610-0394 Japan

出版信息

RSC Adv. 2018 Jul 25;8(47):26571-26579. doi: 10.1039/c8ra05088k. eCollection 2018 Jul 24.

DOI:10.1039/c8ra05088k
PMID:35541036
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9083275/
Abstract

We decorated a single giant DNA (1.66 × 10 base pairs) with gold nanoparticles through the simple procedure of mild warming, without denaturation of the DNA molecule. Single-molecule observation with fluorescence microscopy revealed that individual decorated DNA molecules stay in the bulk solution by avoiding aggregation and precipitation, and exhibit translational and conformational fluctuation, , Brownian motion. An analysis of the intra-chain fluctuation of single DNA molecules revealed that the apparent spring constant and damping coefficient of a DNA chain increased by 13- and 5-fold, respectively, upon decoration with gold nanoparticles. Observation by transmission electron microscopy revealed that gold nanoparticles were stably attached to the DNA skeleton. UV-visible measurements revealed the absence of any detectable change in surface plasmon resonance, suggesting that the gold nanoparticles assemble without the formation of a densely packed aggregate. CD measurements showed that the secondary structure of decorated DNA is still essentially the B-form.

摘要

我们通过温和加热的简单步骤，在不使DNA分子变性的情况下，用金纳米颗粒修饰了单个巨大的DNA（1.66×10个碱基对）。荧光显微镜的单分子观察表明，单个修饰的DNA分子通过避免聚集和沉淀而留在本体溶液中，并表现出平移和构象波动，即布朗运动。对单个DNA分子链内波动的分析表明，在用金纳米颗粒修饰后，DNA链的表观弹簧常数和阻尼系数分别增加了13倍和5倍。透射电子显微镜观察表明，金纳米颗粒稳定地附着在DNA骨架上。紫外可见测量表明表面等离子体共振没有任何可检测到的变化，这表明金纳米颗粒在组装时没有形成紧密堆积的聚集体。圆二色性测量表明，修饰后DNA的二级结构基本上仍为B型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/9083275/f69d7a093f45/c8ra05088k-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/9083275/f23c7b87139d/c8ra05088k-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/9083275/3c97534d1568/c8ra05088k-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/9083275/f2818e4163b8/c8ra05088k-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/9083275/e357a967ccb5/c8ra05088k-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/9083275/ce00ac09d6a5/c8ra05088k-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/9083275/f69d7a093f45/c8ra05088k-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/9083275/f23c7b87139d/c8ra05088k-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/9083275/3c97534d1568/c8ra05088k-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/9083275/f2818e4163b8/c8ra05088k-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/9083275/e357a967ccb5/c8ra05088k-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/9083275/ce00ac09d6a5/c8ra05088k-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0c4c/9083275/f69d7a093f45/c8ra05088k-f6.jpg

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