Ruiz Jimena A, Fernández Rubén O, Nikel Pablo I, Méndez Beatriz S, Pettinari M Julia
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
FEMS Microbiol Lett. 2006 May;258(1):55-60. doi: 10.1111/j.1574-6968.2006.00196.x.
arcA codes for a central regulator in Escherichia coli that responds to redox conditions of growth. Mutations in this gene, originally named dye, confer sensitivity to toluidine blue and other redox dyes. However, the molecular basis for the dye-sensitive phenotype has not been elucidated. In this work, we show that toluidine blue redirects electrons to O2 and causes an increase in the generation of reactive O2 species (ROS). We also demonstrate that synthesis of poly (3-hydroxybutyrate) suppresses the Dye phenotype in E. coli recombinants, as the capacity to synthesize the polymer reduces sensitivity to toluidine blue, O2 consumption and ROS production levels.
arcA编码大肠杆菌中的一种中央调节因子，该调节因子对生长的氧化还原条件作出反应。这个最初名为dye的基因发生突变会导致对甲苯胺蓝和其他氧化还原染料敏感。然而，染料敏感表型的分子基础尚未阐明。在这项研究中，我们表明甲苯胺蓝将电子重定向至O2并导致活性氧（ROS）生成增加。我们还证明，聚（3-羟基丁酸酯）的合成可抑制大肠杆菌重组体中的Dye表型，因为合成该聚合物的能力可降低对甲苯胺蓝的敏感性、O2消耗和ROS产生水平。
