树突启迪：利用图案化双光子解笼技术揭示大脑最小树突的奥秘

Dendritic enlightenment: using patterned two-photon uncaging to reveal the secrets of the brain's smallest dendrites.

作者信息

Judkewitz Benjamin, Roth Arnd, Häusser Michael

机构信息

Wolfson Institute for Biomedical Research and Department of Physiology, University College London, Gower Street, London WC1E 6BT, United Kingdom.

出版信息

Neuron. 2006 Apr 20;50(2):180-3. doi: 10.1016/j.neuron.2006.04.011.

DOI:10.1016/j.neuron.2006.04.011

PMID:16630828

Abstract

It has been a longstanding challenge for experimentalists to manipulate precisely the spatial and temporal patterns of synaptic input to the dendritic tree in order to mimic activity occurring in the intact brain and determine their importance for synaptic integration. In this issue of Neuron, Losonczy and Magee have used rapid multisite two-photon uncaging of glutamate to define patterns of synaptic input on a submillisecond and micron scale to investigate the rules for summation of synaptic inputs in the fine oblique dendrites of pyramidal neurons.

摘要

长期以来，实验人员面临的一个挑战是精确操纵输入到树突的突触输入的空间和时间模式，以模拟完整大脑中发生的活动，并确定其对突触整合的重要性。在本期《神经元》杂志中，洛松奇和马吉利用谷氨酸的快速多部位双光子解笼技术，在亚毫秒和微米尺度上定义突触输入模式，以研究锥体神经元细斜树突中突触输入总和的规则。