Uchida Hideaki, Kinoshita Hideki, Kawai Yasushi, Kitazawa Haruki, Miura Koh, Shiiba Kenichi, Horii Akira, Kimura Katsunori, Taketomo Naoki, Oda Munehiro, Yajima Takaji, Saito Tadao
Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Tsutsumidori-Amamiyamachi 1-1, Aoba-ku, Sendai 981-8555, Miyagi, Japan.
Res Microbiol. 2006 Sep;157(7):659-65. doi: 10.1016/j.resmic.2006.03.001. Epub 2006 Mar 30.
Adherent lactic acid bacteria (LAB) in the human intestine were investigated using the surface plasmon resonance technique with the biosensor BIACORE-1000. Ninety-three LAB strains were isolated from human feces and evaluated for binding to human blood type-A antigen [GalNAcalpha1-3 (Fucalpha1-2) Gal-: A-trisaccharide] expressed in the intestinal mucosa. Eleven strains showed strong adherence to an A-trisaccharide biotinyl polymer (BP) probe, and slightly or no adherence to a B-trisaccharide BP probe. Four strains with high adherence (high A/B ratio) were selected and their surface layer proteins (SLPs) were evaluated for A-antigen ligand binding using BIACORE. The SLP from L. brevis strain OLL2772 showed a single band at ca. 48 kDa by SDS-PAGE analysis and it had a very strong adherence to the human A-antigen, as shown using an anti-A lectin blocking technique. A partial N-terminal sequence of the band showed strong homology to an S-layer protein of L. brevis ATCC8287T. The probiotic LAB binds to human blood type-A antigen expressed in the intestinal mucosa which may aid in colonization of the gut.
采用表面等离子体共振技术，利用BIACORE - 1000生物传感器对人肠道内的黏附性乳酸菌（LAB）进行了研究。从人粪便中分离出93株LAB菌株，并评估它们与在肠黏膜中表达的人A型血抗原[GalNAcalpha1 - 3（Fucalpha1 - 2）Gal -：A - 三糖]的结合情况。11株菌株对A - 三糖生物素聚合物（BP）探针表现出强黏附性，而对B - 三糖BP探针的黏附性较弱或无黏附性。选择了4株黏附性高（高A/B比值）的菌株，并用BIACORE评估它们的表层蛋白（SLP）与A抗原配体的结合情况。短乳杆菌OLL2772菌株的SLP经SDS - PAGE分析在约48 kDa处显示出一条带，并且如使用抗A凝集素阻断技术所示，它对人A抗原具有非常强的黏附性。该条带的部分N端序列与短乳杆菌ATCC8287T的S层蛋白具有高度同源性。益生菌LAB与肠黏膜中表达的人A型血抗原结合，这可能有助于其在肠道内定殖。
