Mori H, Sawairi M, Itoh N, Hanabayashi T, Tamaya T
Department of Obstetrics and Gynecology, Gifu University School of Medicine, Japan.
J In Vitro Fert Embryo Transf. 1991 Dec;8(6):329-35. doi: 10.1007/BF01133023.
The effects of estradiol (E2), progesterone (P), and testosterone (T) on the production of immunoglobulin (Ig) M by Epstein-Barr virus-transformed B-cell line, SKW6-CL4, were investigated. Interleukin 6 (IL-6) induced IgM production by SKW6-CL4 cells in a dose-dependent manner without any significant increase in thymidine incorporation. E2 at concentrations ranging from 10(-10) to 10(-9) M enhanced IL-6-induced IgM production by SKW6-CL4 cells, whereas E2 at a high concentration of 10(-7) M inhibited both the IgM production and the growth of SKW6-CL4 cells. Time-course studies revealed that E2 acts in the early phase of differentiation of SKW6-CL4 cells in response to IL-6. On the other hand, P and T at physiological and superphysiological levels did not influence either the IgM production or the proliferation of SKW6-CL4 cells. These findings suggest a direct immunoregulatory effect of E2 on human B lineage cells and support the concept that E2 may have a role in the pathogenesis of some autoimmune diseases.
研究了雌二醇（E2）、孕酮（P）和睾酮（T）对爱泼斯坦-巴尔病毒转化的B细胞系SKW6-CL4产生免疫球蛋白（Ig）M的影响。白细胞介素6（IL-6）以剂量依赖的方式诱导SKW6-CL4细胞产生IgM，而胸苷掺入没有任何显著增加。浓度范围为10（-10）至10（-9）M的E2增强了IL-6诱导的SKW6-CL4细胞IgM产生，而高浓度10（-7）M的E2则抑制了SKW6-CL4细胞的IgM产生和生长。时间进程研究表明，E2在SKW6-CL4细胞响应IL-6的分化早期起作用。另一方面，生理和超生理水平的P和T对SKW6-CL4细胞的IgM产生或增殖均无影响。这些发现提示E2对人B淋巴细胞系具有直接的免疫调节作用，并支持E2可能在某些自身免疫性疾病发病机制中起作用的观点。
