Yamaguchi T
Department of Urology, Akita University School of Medicine.
Nihon Hinyokika Gakkai Zasshi. 1991 Oct;82(10):1642-8. doi: 10.5980/jpnjurol1989.82.1642.
I have investigated the effects of autonomic drugs and prostaglandins on in vitro smooth muscle spontaneous contractions and made the quantitative analysis of autonomic receptors in the canine ureter. Ureteral muscle strip cut helically usually generated spontaneous contractions whereas those cut circularly or longitudinally did not generate spontaneous contractions. These results suggest the importance of knowing which direction to cut the ureteral smooth muscle in order to generate spontaneous contractions. Norepinephrine (alpha), phenylephrine (alpha 1), carbachol (muscarinic) and PGF2 alpha caused significant increase in contractile force. Terbutaline (beta 2) and PGE2 caused significant decreases in contractile force, while dobutamine (beta 1) and clonidine (alpha) caused no effect. Autonomic receptor densities were determined using radiology and binding techniques. The number of maximum binding sites (Bmax) of 3H-prazosin (PZ), 3H-yohimbine (YOH), 3H-dihydroaloprenolol (DHA) and 3H-quinuclidinylbenzilate (QNB) were 53.8, 16.9, 11.2 and 5.28 fmol/ml protein, respectively. These data suggest that the contractile responses to adrenergic and cholinergic agonists in the canine ureter are mediated through functional adrenergic (alpha 1, beta 2) and muscarinic cholinergic receptors and that the prostaglandins have a role in the contraction of the canine ureter.
我研究了自主神经药物和前列腺素对体外平滑肌自发收缩的影响，并对犬输尿管中的自主神经受体进行了定量分析。螺旋形切割的输尿管肌条通常会产生自发收缩，而圆形或纵向切割的肌条则不会产生自发收缩。这些结果表明，了解切割输尿管平滑肌的方向对于产生自发收缩非常重要。去甲肾上腺素（α）、去氧肾上腺素（α1）、卡巴胆碱（毒蕈碱型）和前列腺素F2α可使收缩力显著增加。特布他林（β2）和前列腺素E2可使收缩力显著降低，而多巴酚丁胺（β1）和可乐定（α）则无影响。使用放射学和结合技术测定自主神经受体密度。3H-哌唑嗪（PZ）、3H-育亨宾（YOH）、3H-二氢阿普洛尔（DHA）和3H-喹核醇基苯甲酸酯（QNB）的最大结合位点数（Bmax）分别为53.8、16.9、11.2和5.28 fmol/ml蛋白质。这些数据表明，犬输尿管对肾上腺素能和胆碱能激动剂的收缩反应是通过功能性肾上腺素能（α1、β2）和毒蕈碱型胆碱能受体介导的，并且前列腺素在犬输尿管的收缩中起作用。
