Ogunro P S, Ogungbamigbe T O, Ajala M O, Egbewale B E
Department of Chemical Pathology, College of Health Sciences, Ladoke Akintola University of Technology, Osogbo, Nigeria.
Afr J Med Med Sci. 2005 Sep;34(3):221-5.
Total serum antioxidant status (TAS) and lipid peroxidation were evaluated in 62 HIV-1 seropositive patients before the commencement of antiretroviral drug therapy. Twenty-four (24) age-marched and apparently healthy HIV-1 seronegative subjects were recruited as control subjects. HIV-1 seropositive patients were classified according to the Centre for Disease Control and Prevention (CDC) Criteria. The mean serum malondialdehyde (MDA) concentrations of 5.58 +/- 0.99nmol/ml and 4.24 +/- 0.80nmol/ml were significantly elevated in HIV-1 infected patients with CD4+ count of <200 lym/mm3 and that of 200-499 lym/mm3 respectively when compared with the control which is 3.37 +/- 0.56nmol/ ml (P<0.001). Whereas when compared with control, the serum MDA concentration of 3.45 +/- 0.48nmol/ml in HIV-1 patients with CD4+ count of >500 lym/mm3 was not significant (P>0.05). In the intra groups comparison serum concentration of MDA were significantly elevated when compared with each other (P<0.001). The mean serum total antioxidant status (TAS) of 1.30 +/- 0.11mmol/L, 1.12 +/- 0.24 mmol/L and 0.95 +/- 0.17mmol/L of HIV- 1 seropositive patients with CD4+ count of >500 lym/mm3, 200-499 lym/mm3 and <200 lym/mm3 respectively were significantly reduced compared with 1.69 +/- 0.23mmol/L for the control group (P<0.001). Similarly in the intra groups comparison, serum concentration of TAS were significantly reduced when compared with each other (P<0.001). The weight of 54.81 +/- 5.13 Kg for HIV-I seropositive patients with CD4+ count of <200 lym/mm3 was significantly reduced when compared with 69.17 +/- 4.38Kg for the control (P<0.05). Our results clearly show that severe oxidative stress occurs in the serum of seropositive patients in comparison with controls, and increases significantly with the progression of disease, i.e. AIDS > symptomatics > asymptomatics > controls. The inclusion of antioxidants in the therapeutic approach in managing HIV- 1 seropositive patients will prevent the additional damage that free radicals could do to such patients.
在62例接受抗逆转录病毒药物治疗前的HIV-1血清阳性患者中评估了总血清抗氧化状态（TAS）和脂质过氧化情况。招募了24名年龄匹配且表面健康的HIV-1血清阴性受试者作为对照。HIV-1血清阳性患者根据疾病控制与预防中心（CDC）标准进行分类。与对照组（3.37±0.56nmol/ml）相比，CD4+细胞计数<200个/μl和200 - 499个/μl的HIV-1感染患者的平均血清丙二醛（MDA）浓度分别显著升高至5.58±0.99nmol/ml和4.24±0.80nmol/ml（P<0.001）。而与对照组相比，CD4+细胞计数>500个/μl的HIV-1患者血清MDA浓度为3.45±0.48nmol/ml，差异无统计学意义（P>0.05）。在组内比较中，血清MDA浓度相互比较时显著升高（P<0.001）。CD4+细胞计数>500个/μl、200 - 499个/μl和<200个/μl的HIV-1血清阳性患者的平均血清总抗氧化状态（TAS）分别为1.30±0.11mmol/L、1.12±0.24mmol/L和0.95±0.17mmol/L，与对照组的1.69±0.23mmol/L相比显著降低（P<0.001）。同样在组内比较中，血清TAS浓度相互比较时显著降低（P<0.001）。CD4+细胞计数<200个/μl的HIV-1血清阳性患者体重为54.81±5.13kg，与对照组的69.17±4.38kg相比显著降低（P<0.05）。我们的结果清楚地表明，与对照组相比，血清阳性患者血清中发生严重氧化应激，且随着疾病进展即艾滋病>有症状者>无症状者>对照组显著增加。在治疗HIV-1血清阳性患者的方法中加入抗氧化剂将防止自由基对这类患者造成额外损害。
