Barrado E, Rodríguez J A, Castrillejo Y
Departamento de Química Analítica, Facultad de Ciencias, Universidad de Valladolid, Paseo Prado de la Magdalena s/n, 47005, Valladolid, Spain.
Anal Bioanal Chem. 2006 Aug;385(7):1233-40. doi: 10.1007/s00216-006-0356-y. Epub 2006 Apr 22.
A new chromatographic modality that does not require high pressures and also allows renewal of the stationary phase as desired is reported. The technique is based on a thin layer paramagnetic stationary phase (Fe3O4-SiO2) retained on the inner wall of a minicolumn through the action of an external magnetic field, which also plays an important role in separating the analytes. Accordingly, the name "renewable stationary phase liquid magnetochromatography", or RSP-LMC, has been proposed for it. The technique was used to separate and quantify the sugar substitute alpha-aspartame and its constituent amino acids (hydrolysis products), L-aspartic acid and L-phenylalanine, in diet fizzy soft drinks. When the results obtained for alpha-aspartame were compared with those obtained using HPLC as a reference method, no significant differences were observed. The system proposed is fully automated, making it an economic, competitive alternative to conventional methods of determining alpha-aspartame and its amino acid components.
据报道，一种新型色谱方法，它不需要高压，并且能够根据需要更新固定相。该技术基于通过外部磁场作用保留在微型柱内壁上的薄层顺磁性固定相（Fe3O4-SiO2），这在分离分析物方面也起着重要作用。因此，已为其提出了“可再生固定相液体磁色谱法”，即RSP-LMC这一名称。该技术用于分离和定量低糖碳酸软饮料中的甜味剂α-阿斯巴甜及其组成氨基酸（水解产物），L-天冬氨酸和L-苯丙氨酸。当将α-阿斯巴甜获得的结果与使用HPLC作为参考方法获得的结果进行比较时，未观察到显著差异。所提出的系统是完全自动化的，使其成为测定α-阿斯巴甜及其氨基酸成分的传统方法的经济且有竞争力的替代方法。
