Koçkaya E Arzu, Akay M Turan
Department of Biology, Faculty of Science, Hacettepe University, 06532, Beytepe, Ankara, Türkiye.
Cell Biochem Funct. 2006 May-Jun;24(3):223-7. doi: 10.1002/cbf.1215.
In this study, immunolocalization of tenascin (TN), type IV collagen and histopathological changes in the liver of fetuses of pregnant Wistar albino rats treated with two doses of alcohol (1 and 5 g kg(-1) day(-1)) were determined. The samples of liver obtained from fetuses of rats on day 21 of gestation were evaluated morphologically and fixed for histology and immunohistochemistry. The sections stained with H&E and peroxidase-antiperoxidase (PAP) method for TN and type IV collagen were evaluated and immunohistochemical staining results were examined by the intensity of labelling. Statistical evaluation of data showed that there were no differences in liver weights between the control and calorie control groups; but some differences in relative liver weight between the control and alcohol treatment groups were determined. The changes in the liver histology such as degeneration of the cytoplasm of hepatocytes and increases in the number of megakaryocytes were seen in the fetuses of groups receiving alcohol during pregnancy. Increases in the immunolocalization of TN and type IV collagen in the liver of fetuses of alcohol-receiving rats were also found.
在本研究中，测定了用两剂量酒精（1和5 g kg⁻¹ 天⁻¹）处理的怀孕Wistar白化大鼠胎儿肝脏中肌腱蛋白（TN）、IV型胶原的免疫定位以及组织病理学变化。从妊娠第21天的大鼠胎儿获取肝脏样本，进行形态学评估，并固定用于组织学和免疫组织化学检查。对用苏木精-伊红（H&E）染色以及用过氧化物酶-抗过氧化物酶（PAP）法对TN和IV型胶原染色的切片进行评估，并通过标记强度检查免疫组织化学染色结果。数据的统计学评估表明，对照组和热量对照组之间肝脏重量无差异；但对照组和酒精处理组之间相对肝脏重量存在一些差异。在孕期接受酒精的组的胎儿中可见肝脏组织学变化，如肝细胞细胞质变性和巨核细胞数量增加。还发现接受酒精的大鼠胎儿肝脏中TN和IV型胶原的免疫定位增加。
