Turan Akay Mehmet, Arzu Koçkaya Evrim
Department of Biology, Faculty of Science, Hacettepe University, 06532 Beytepe, Ankara, Türkiye.
Cell Biochem Funct. 2005 Nov-Dec;23(6):435-45. doi: 10.1002/cbf.1175.
In this study, daily food and water consumption and body weights, histopathology of placenta, tenascin (TN), type IV collagen and EGF and its receptor immunolocalization in the placenta of albino rats treated with two doses of alcohol (1 and 5 g kg(-1) day(-1)) were determined. Alcohol was administered in three different periods i.e. the whole 4 weeks before the pregnancy, during the pregnancy, and during the 4 weeks before the pregnancy plus pregnancy itself. The samples of placenta obtained from control and treated rats on days 10, 12, 14, 16, 18, 20 and 21 of gestation were evaluated morphologically and fixed for histology and immunohistochemistry. Some differences in food and water consumption between the groups were determined. The placental weight, especially in the groups receiving 1 and 5 g kg(-1) day(-1) alcohol during the pregnancy, showed increases. The changes in placental histology such as increases in the number and the size of trophoblastic giant cells, cytoplasmic dissolution and nuclear polymorphism, degenerations in spongiotrophoblasts, hyperemia at the basal zone and labyrinth, hyperplasia at the labyrinth and irregular vascularization were seen particularly in the groups receiving alcohol during the pregnancy, and during the 4 weeks before the pregnancy plus pregnancy itself. Increases in the immunolocalization of TN and type IV collagen and decreases in the immunolocalization of EGF and EGFR in the placentas of alcohol-receiving rats were found. In conclusion, ethanol treatment during pregnancy in rats affected placentation and the immunolocalization of TN, type IV collagen, EGF and EGFR in the placentas.
在本研究中，测定了给予两剂量酒精（1和5 g kg⁻¹ 天⁻¹）处理的白化大鼠胎盘的每日食物和水消耗量、体重、组织病理学、腱生蛋白（TN）、IV型胶原、表皮生长因子（EGF）及其受体的免疫定位。酒精在三个不同时期给予，即怀孕前整个4周、怀孕期间以及怀孕前4周加怀孕期本身。在妊娠第10、12、14、16、18、20和21天从对照大鼠和处理大鼠获取胎盘样本，进行形态学评估，并固定用于组织学和免疫组织化学检查。确定了各组之间在食物和水消耗量方面的一些差异。胎盘重量增加，尤其是在怀孕期间接受1和5 g kg⁻¹ 天⁻¹酒精处理的组。胎盘组织学变化，如滋养层巨细胞数量和大小增加、细胞质溶解和核多态性、海绵滋养层变性、基底层和迷路充血、迷路增生以及血管形成不规则，尤其在怀孕期间以及怀孕前4周加怀孕期本身接受酒精处理的组中可见。在接受酒精处理的大鼠胎盘中发现TN和IV型胶原免疫定位增加，而EGF和EGFR免疫定位减少。总之，大鼠孕期乙醇处理影响胎盘形成以及胎盘中TN、IV型胶原、EGF和EGFR的免疫定位。
