Li Baozhi, Bai Jie, Yang Gengliang, Li Zhiwei, Wang Lijuan, Chen Yi
College of Pharmacy, Hebei University, Baoding 071002, People's Republic of China.
Phytochem Anal. 2006 Mar-Apr;17(2):129-33. doi: 10.1002/pca.896.
A new method has been established to convert triptolide (1) into tripchlorolide (2) directly in the Chinese herbal drug Tripterygium wilfordii Hook F. and the influences of reaction times and pH values have been investigated. It was found that 1 could be most efficiently converted into 2 using a hydrochloric acid-acetic acid system. An HPLC method was devised in order to monitor the conversion and ESI-MS was used to identify the synthetic product 2. The sensitivity of the assay was sufficient to monitor the conversion of the main active components in T. wilfordii.
已建立一种新方法，可直接在中药雷公藤中把雷公藤内酯醇（1）转化为雷公藤氯内酯醇（2），并研究了反应时间和pH值的影响。结果发现，使用盐酸 - 乙酸体系可将1最有效地转化为2。设计了一种高效液相色谱法来监测该转化过程，并使用电喷雾质谱法鉴定合成产物2。该测定方法的灵敏度足以监测雷公藤中主要活性成分的转化情况。
