Psathakis K, Mermigkis D, Papatheodorou G, Loukides S, Panagou P, Polychronopoulos V, Siafakas N M, Bouros D
Department of Pneumonology and the Clinical Research Unit, Army General Hospital of Athens, and Department of Pneumonology, University Hospital of Heraklion, Greece.
Eur J Clin Invest. 2006 May;36(5):362-7. doi: 10.1111/j.1365-2362.2006.01636.x.
Expired breath condensate (EBC) has never been used to explore the level of oxidative stress in idiopathic pulmonary fibrosis (IPF). Therefore, the aim of this study was to measure the levels of H2O2 and 8-isoprostane, as biomarkers of oxidative stress, in the EBC of patients with IPF.
We investigated 16 patients with IPF and 15 healthy subjects as the control group. The levels of H2O2 and 8-isoprostane were measured in the EBC of all subjects and were compared between the IPF and control groups. In patients with IPF, H2O2 and 8-isoprostane were further correlated with pulmonary function tests (PFTs), the resting pO2 and the differential cell count from the bronchoalveolar lavage fluid (BALF).
The mean (95%CI) concentration of H2O2 was increased in the patients with IPF compared with the normal subjects (0.36, 0.24-0.47 microM vs. 0.16, 0.10-0.23 microM, P=0.003). The mean (95%CI) concentration of 8-isoprostane was also increased in the patients with IPF compared with the controls (74, 38-110 pg mL-1 vs. 33, 28-39 pg mL-1, P=0.02). In the patients with IPF, the diffusing capacity of the lung for carbon monoxide was negatively correlated with the levels of H2O2 in EBC (P=0.03, r=-0.58). No other correlation was found between the oxidative stress markers in the EBC and PFT values, pO2 or BALF cell count.
Our data suggest that H2O2 and 8-isoprostane are increased in the EBC of patients with IPF. H2O2 may be correlated with the severity of the disease in IPF.
呼出气冷凝液（EBC）从未被用于探究特发性肺纤维化（IPF）中的氧化应激水平。因此，本研究的目的是测量IPF患者EBC中作为氧化应激生物标志物的过氧化氢（H2O2）和8-异前列腺素的水平。
我们调查了16例IPF患者和15名健康受试者作为对照组。测量了所有受试者EBC中H2O2和8-异前列腺素的水平，并在IPF组和对照组之间进行了比较。在IPF患者中，H2O2和8-异前列腺素进一步与肺功能测试（PFT）、静息氧分压（pO2）以及支气管肺泡灌洗（BALF）液中的细胞分类计数相关。
与正常受试者相比，IPF患者中H2O2的平均（95%可信区间）浓度升高（0.36，0.24 - 0.47微摩尔/升 对比 0.16，0.10 - 0.23微摩尔/升，P = 0.003）。与对照组相比，IPF患者中8-异前列腺素的平均（95%可信区间）浓度也升高（74，38 - 110皮克/毫升 对比 33，28 - 39皮克/毫升，P = 0.02）。在IPF患者中，肺一氧化碳弥散量与EBC中H2O2水平呈负相关（P = 0.03，r = -0.58）。在EBC中的氧化应激标志物与PFT值、pO2或BALF细胞计数之间未发现其他相关性。
我们的数据表明，IPF患者的EBC中H2O2和8-异前列腺素升高。H2O2可能与IPF疾病的严重程度相关。
