Corradi Massimo, Pignatti Patrizia, Brunetti Giuseppe, Goldoni Matteo, Caglieri Andrea, Nava Stefano, Moscato Gianna, Balbi Bruno
Department of Clinical Medicine, Nephrology and Health Sciences, University of Parma, Parma, Italy.
Acta Biomed. 2008;79 Suppl 1:73-8.
The aim of the present study was to compare hydrogen peroxide (H2O2) levels resulting from oxidative stress in exhaled breath condensate (EBC) and bronchoalveolar lavage (BAL) supernatants of subjects with different diffuse interstitial lung diseases (DILDs). Twenty-one patients who underwent BAL procedure for various DILDs were studied. EBC, which was collected the day before bronchoscopy, was obtained by cooling exhaled breath; BAL was processed for differential cellular count and supernatant was stored. H2O2 in both fluids was measured using a commercial fluorimetric kit with a limit of detection of 0.01 microM. No difference in H2O2 levels in EBC and in BAL was observed [median (range), 0.07 microM (0.01-0.6) and 0.08 microM (0.01-0.8), respectively]. No correlation was observed between BAL and EBC levels. H2O2 in BAL did not correlate with differential cellular count, whereas H2O2 EBC did correlate positively with the percentage of epithelial cells (r = 0.5, p = 0.007) and negatively with the number of macrophages (r = -0.4, p = 0.03). No correlation was observed between H2O2 levels either in EBC or BAL and lung function data. The data show that in DILDs H2O2 can be detected in both EBC and supernatants of BAL and that their relative concentrations are similar but not correlated with each other. The positive correlation between H2O2 levels in EBC and percentage of epithelial cells leads to the speculation that airway epithelia may play a relevant contribution in H2O2 production in the airway lumen.
本研究的目的是比较不同弥漫性间质性肺疾病（DILDs）患者呼出气冷凝液（EBC）和支气管肺泡灌洗（BAL）上清液中氧化应激产生的过氧化氢（H2O2）水平。对21例因各种DILDs接受BAL手术的患者进行了研究。在支气管镜检查前一天收集的EBC，通过冷却呼出气获得；对BAL进行细胞分类计数处理，并保存上清液。使用商业荧光试剂盒测量两种液体中的H2O2，检测限为0.01微摩尔。未观察到EBC和BAL中H2O2水平的差异[中位数（范围）分别为0.07微摩尔（0.01 - 0.6）和0.08微摩尔（0.01 - 0.8）]。未观察到BAL和EBC水平之间的相关性。BAL中的H2O2与细胞分类计数无关，而EBC中的H2O2与上皮细胞百分比呈正相关（r = 0.5，p = 0.007），与巨噬细胞数量呈负相关（r = -0.4，p = 0.03）。未观察到EBC或BAL中的H2O2水平与肺功能数据之间的相关性。数据表明，在DILDs中，EBC和BAL上清液中均可检测到H2O2，且它们的相对浓度相似，但彼此不相关。EBC中H2O2水平与上皮细胞百分比之间的正相关导致推测气道上皮可能在气道腔内H2O2的产生中起相关作用。
