Yogeeta Surinder Kumar, Gnanapragasam Arunachalam, Kumar Subramanian Senthil, Subhashini Rajakannu, Sathivel Arumugam, Devaki Thiruvengadum
Department of Biochemistry, University of Madras, Guindy Campus, Chennai, Tamilnadu, India.
Mol Cell Biochem. 2006 Feb;283(1-2):139-46. doi: 10.1007/s11010-006-2494-0.
Studies on the lipid peroxidation and antioxidant changes and their significance during myocardial injury have provided a new insight into the pathogenesis of heart disease. The heart failure subsequent to myocardial infarction may be associated with an antioxidant deficit as well as increased myocardial oxidative stress. The present study was designed to evaluate the effect of the combination of ferulic acid and ascorbic acid on antioxidant defense system and lipid peroxidation against isoproterenol (ISO)-induced myocardial infarction in rats. Induction of rats with isoproterenol (150 mg/kg body weight daily, i.p.) for 2 days resulted in a marked elevation in lipid peroxidation, serum marker enzymes (LDH, CPK, GOT, and GPT), and a significant decrease in activities of endogenous antioxidants (SOD, GPx, GST, CAT, and GSH). Pre-co-treatment with the combination of ferulic acid (20 mg/kg body weight/day) and ascorbic acid (80 mg/kg body weight/day) orally for 6 days, significantly attenuated these changes when compared to the individual treatment groups. Histopathological observations were also in correlation with the biochemical parameters. Thus, ferulic acid and ascorbic acid significantly counteracted the pronounced oxidative stress effect of ISO by the inhibition of lipid peroxidation, restoration of antioxidant status, and myocardial marker enzymes levels. In conclusion, these findings indicate the synergistic protective effect of ferulic acid and ascorbic acid on lipid peroxidation and antioxidant defense system during ISO-induced myocardial infarction and associated oxidative stress in rats.
关于心肌损伤期间脂质过氧化和抗氧化剂变化及其意义的研究为心脏病发病机制提供了新的见解。心肌梗死后的心力衰竭可能与抗氧化剂缺乏以及心肌氧化应激增加有关。本研究旨在评估阿魏酸和抗坏血酸联合使用对大鼠异丙肾上腺素（ISO）诱导的心肌梗死的抗氧化防御系统和脂质过氧化的影响。用异丙肾上腺素（每天150mg/kg体重，腹腔注射）诱导大鼠2天，导致脂质过氧化、血清标志物酶（乳酸脱氢酶、肌酸磷酸激酶、谷草转氨酶和谷丙转氨酶）显著升高，内源性抗氧化剂（超氧化物歧化酶、谷胱甘肽过氧化物酶、谷胱甘肽S-转移酶、过氧化氢酶和谷胱甘肽）活性显著降低。与单独治疗组相比，预先口服阿魏酸（20mg/kg体重/天）和抗坏血酸（80mg/kg体重/天）联合用药6天，可显著减轻这些变化。组织病理学观察结果也与生化参数相关。因此，阿魏酸和抗坏血酸通过抑制脂质过氧化、恢复抗氧化状态和心肌标志物酶水平，显著抵消了ISO的明显氧化应激作用。总之，这些发现表明阿魏酸和抗坏血酸对大鼠ISO诱导的心肌梗死及相关氧化应激期间的脂质过氧化和抗氧化防御系统具有协同保护作用。
