Matsukawa Kazushige, Ogata Mika, Hikage Takashi, Minami Hiroyuki, Shimotai Yoshitaka, Saitoh Yasushi, Yamashita Tetsuro, Ouchi Atsushi, Tsutsumi Reiko, Fujioka Tomoaki, Tsutsumi Ken-Ichi
Cryobiosystem Research Center, Iwate University, Japan.
Biosci Biotechnol Biochem. 2006 Apr;70(4):1046-8. doi: 10.1271/bbb.70.1046.
We describe a novel pharmacological activity of the gentian root, an ingredient of Chinese medicines. Root extract from Gentiana triflora triggered cell death of human Daudi cells in culture. In addition, daily administration of the extract to mice inhibited growth of implanted solid tumors. Extract treatment of cultured cells resulted in the appearance of shranken, fragmented, or condensed cell and nuclear morphologies, and in chromosomal DNA degradation. But, the extract-treated cells did not show DNA fragmentation, which exhibits a nucleosome ladder, suggesting that extract-triggered cell death is not mediated through a typical apoptotic pathway.
我们描述了龙胆根（一种中药成分）的一种新的药理活性。三花龙胆的根提取物在培养中引发了人Daudi细胞的死亡。此外，每天给小鼠施用该提取物可抑制植入实体瘤的生长。对培养细胞进行提取物处理导致细胞和细胞核形态出现收缩、碎片化或凝聚，以及染色体DNA降解。但是，经提取物处理的细胞未显示出呈现核小体梯状条带的DNA片段化，这表明提取物引发的细胞死亡不是通过典型的凋亡途径介导的。
