Fontanilla J M, Montes M, De Prado R
Departamento de Química Agrícola y Edafología, Universidad de Córdoba, España.
Commun Agric Appl Biol Sci. 2005;70(3):35-40.
Harpin(Ea), an active component in Messenger, was originally isolated from the bacterial plant pathogen Erwinia amylovora based on its ability to elicit a hypersensitive response (HR). This bacterial pathogen causes "fire blight", a disease in apples and other members of the Rosaceae. Harpin(Ea) also induces resistance in a variety of plants against a wide array of pathogens. The objective of this investigation was to determine indications of resistance induction to Botrytis cinerea in the plants treated with the formulated Harpin(Ea) protein. This pathogenic agent is responsible for important economic losses in the cultivation of both greenhouse and field grown tomatoes. Plants with and without Messenger applications were inoculated with Botrytis cinerea in the same way. In addition, some plants with and without Messenger applications were not inoculated. Inoculated plants were symptomatologically checked for local and systemic symptoms. Evaluations of the number of tomatoes produced and their growth were also carried out. The results obtained demonstrate that there were active defence systems in the plant, producing a reduction in the damage caused by the pathogenic agent Botrytis cinerea. A greater growth also took place in the fruit obtained. These results show that the formulated Harpin(Ea) protein could reduce the use of chemical agents and be a new tool to serve as the basis for an Integrated Pest Management system.
Harpin（Ea）是Messenger中的一种活性成分，最初是从细菌性植物病原体梨火疫病菌中分离出来的，基于其引发超敏反应（HR）的能力。这种细菌病原体导致“火疫病”，一种在苹果和蔷薇科其他植物中的病害。Harpin（Ea）还能诱导多种植物对多种病原体产生抗性。本研究的目的是确定用配方化的Harpin（Ea）蛋白处理过的植物对灰葡萄孢菌诱导抗性的迹象。这种病原体在温室和田间种植的番茄栽培中造成了重大经济损失。对使用和未使用Messenger的植物以相同方式接种灰葡萄孢菌。此外，对一些使用和未使用Messenger的植物不进行接种。对接种的植物进行症状学检查，以查看局部和全身症状。还对所产番茄的数量及其生长情况进行了评估。所获得的结果表明，植物中存在活跃的防御系统，减少了由病原体灰葡萄孢菌造成的损害。所收获的果实也有更大的生长。这些结果表明，配方化的Harpin（Ea）蛋白可以减少化学药剂的使用，并成为综合虫害管理系统基础的一种新工具。
