Costello C B, Jasani B, Kumar S
Burnley General Hospital, U.K.
Anticancer Res. 1991 Nov-Dec;11(6):2159-61.
Tamm-Horsfall Protein (THP) is found to be localised in the ascending loop of Henle and the distal tubules of normal and foetal kidneys. We have used an anti-THP monoclonal antibody (D2) to stain foetal, normal and kidney tumours. The dinitrophenyl hapten staining technique was the most specific and sensitive staining procedure. The staining of Tamm-Horsfall Protein in foetal and normal kidney was in agreement with most other investigators. The majority of renal cell carcinoma (22/24) and rhabdoid renal tumours (3/4) stained positively for THP. None of the Wilms' tumours, mesoblastic nephromas or bone metastasising renal tumours of childhood gave positive staining. The findings would suggest that renal cell carcinomas and rhabdoid kidney tumours arise from ascending loop of Henle or distal tubules. The lack of positive staining in the majority of childhood renal tumours raises the possibility of a different histogenesis for these neoplasms.
发现Tamm-Horsfall蛋白（THP）定位于正常和胎儿肾脏的髓袢升支和远曲小管。我们使用抗THP单克隆抗体（D2）对胎儿、正常肾脏及肾脏肿瘤进行染色。二硝基苯基半抗原染色技术是最特异和敏感的染色方法。胎儿和正常肾脏中Tamm-Horsfall蛋白的染色结果与大多数其他研究者一致。大多数肾细胞癌（22/24）和横纹肌样肾肿瘤（3/4）THP染色呈阳性。儿童期的肾母细胞瘤、中胚叶肾瘤或骨转移性肾肿瘤均无阳性染色。这些发现提示肾细胞癌和横纹肌样肾肿瘤起源于髓袢升支或远曲小管。大多数儿童期肾肿瘤缺乏阳性染色，提示这些肿瘤可能具有不同的组织发生学。
