Kumar S, Marsden H B, Jasani B, Kumar P
Christie Hospital, Manchester.
J Clin Pathol. 1987 Dec;40(12):1456-62. doi: 10.1136/jcp.40.12.1456.
A monoclonal antibody to Tamm-Horsfall glycoprotein was used for the immuno-localization of Tamm-Horsfall protein in formalin fixed, paraffin embedded tissue sections of childhood renal tumours, normal children's kidneys, and human fetal kidneys. The procedure was a dinitrophenyl hapten sandwich staining method. The antibody, diluted 1/100,000, gave a very strong and specific staining of the loop of Henle and distal tubules of normal and fetal kidneys. No staining was seen in Wilms' tumour, mesoblastic nephroma, and bone metastasizing renal tumour of childhood. In contrast, two of seven renal carcinomas and three of four rhabdoid renal tumours were positive for Tamm-Horsfall protein.
一种针对Tamm-Horsfall糖蛋白的单克隆抗体被用于在福尔马林固定、石蜡包埋的儿童肾肿瘤、正常儿童肾脏及人胎儿肾脏组织切片中对Tamm-Horsfall蛋白进行免疫定位。该方法为二硝基苯基半抗原夹心染色法。稀释至1/100,000的抗体对正常及胎儿肾脏的髓袢和远端小管产生非常强烈且特异的染色。在儿童肾母细胞瘤、中胚叶肾瘤及骨转移性肾肿瘤中未观察到染色。相比之下，7例肾癌中的2例及4例横纹肌样肾肿瘤中的3例Tamm-Horsfall蛋白呈阳性。
