Howie A J, Smithson N, Raafat F
University of Birmingham, Department of Pathology, Medical School, Birmingham, UK.
Virchows Arch A Pathol Anat Histopathol. 1993;422(5):361-5. doi: 10.1007/BF01605454.
Sections of 114 renal neoplasms from adults, and 2 renal rhabdoid tumours from children, were examined by an indirect immunoperoxidase method using two antibodies to Tamm-Horsfall protein. Forty-five of the adult neoplasms were also examined with an antibody to proximal tubular brush border. Tamm-Horsfall protein is normally only found in the cells of the thick limb of the loop of Henle, and there are widely divergent reports on its occurrence in renal neoplasms. In the present series, Tamm-Horsfall protein was detected in parts of 31 neoplasms. Four distinctive patterns of cell contained the protein: cells with a paranuclear inclusion typical of rhabdoid tumours; 'plasma rich' cells, which were large cells with cytoplasm that was centrally dense and peripherally clear; eosinophilic cells forming one type of papillary structure; and giant cells. The areas containing Tamm-Horsfall protein did not express markers of proximal tubular brush border, and appeared white to the naked eye, rather than the yellow of typical clear cell carcinomas. Tamm-Horsfall protein can therefore be found in renal neoplasms. The four distinctive patterns of positive cells appear to represent neoplastic phenotypes of thick limb cells. This has implications for the classification of renal neoplasms and for theories of their origin.
采用两种抗Tamm-Horsfall蛋白抗体的间接免疫过氧化物酶法，对114例成人肾肿瘤组织切片以及2例儿童肾横纹肌样瘤组织切片进行了检查。对其中45例成人肿瘤组织还使用了一种抗近端肾小管刷状缘的抗体进行检测。Tamm-Horsfall蛋白通常仅在髓袢升支粗段细胞中发现，关于其在肾肿瘤中的出现情况，有多种不同的报道。在本研究系列中，在31例肿瘤的部分区域检测到了Tamm-Horsfall蛋白。含有该蛋白的细胞有四种独特模式：具有横纹肌样瘤典型核旁包涵体的细胞；“富含血浆”细胞，即胞质中央致密、周边清晰的大细胞；形成一种乳头结构的嗜酸性细胞；以及巨细胞。含有Tamm-Horsfall蛋白的区域不表达近端肾小管刷状缘标志物，肉眼观察呈白色，而非典型透明细胞癌的黄色。因此，Tamm-Horsfall蛋白可在肾肿瘤中发现。阳性细胞的四种独特模式似乎代表了髓袢升支粗段细胞的肿瘤表型。这对肾肿瘤的分类及其起源理论具有重要意义。
