Damadi S M, Mosahebi G H, Okhovvat M
Plant Protection of Tehran, Karaj, Iran.
Commun Agric Appl Biol Sci. 2005;70(3):427-9.
Potato virus X (PVX) isolated from the potato leaf and tuber samples which were collected from various fields in Damavand and Ardabil. The initial isolations of the virus were made from potato by mechanical inoculation on Gomphrena globosa L. and Chenopodium spp. that produce local lesion, and then it causes mosaic on Nicotiana spp. and Datura stramonium L. An isolate of the virus inoculated to Nicotiana glutinosa L. and it was maintained throughout the work. Sap from infected N. glutinosa was ineffective after dilution to 10-6, 10 minutes at 70 degrees and 10 weeks at room temperature. The virus was readily purified from infected leaves and the best protocol was Moreira & Jones 1980 than the other 2 methods of Fribourg 1975 and Shepard & Shalla 1972. Antisera were prepared against native, degraded proteins and micro precipitin test showed that both antisera had a 1/512 titer. Precipitin lines with D - Protein antiserum was better of the native protein antiserum in agar double diffusion test than treated with SDS. The isolate of the virus was not transmitted by none of 2 species of Cuscuta but transmitted from infected leaves to healthy plants with sap inoculation without using Carburandum. This isolate showed positive reaction with gamaglubulin in kate received from CIP centre.
从德马万德和阿尔达比勒不同田地采集的马铃薯叶片和块茎样本中分离出马铃薯X病毒（PVX）。最初通过机械接种将该病毒从马铃薯接种到能产生局部病斑的千日红和藜属植物上，然后它会在烟草属植物和曼陀罗上引起花叶病。将该病毒的一个分离株接种到黏毛烟草上，并在整个实验过程中进行保存。感染黏毛烟草的汁液在稀释至10^-6、70摄氏度处理10分钟以及室温下放置10周后均失去活性。该病毒很容易从感染的叶片中纯化出来，与1975年弗里堡和1972年谢泼德及沙拉的另外两种方法相比，莫雷拉和琼斯1980年的方法是最佳方案。针对天然蛋白、降解蛋白制备了抗血清，微量沉淀试验表明两种抗血清的效价均为1/512。在琼脂双扩散试验中，与D - 蛋白抗血清产生的沉淀线比天然蛋白抗血清在经SDS处理后的效果更好。该病毒分离株不能通过两种菟丝子属植物传播，但不使用金刚砂通过汁液接种就能将其从感染叶片传播到健康植株。该分离株在从国际马铃薯中心收到的凯特中与γ球蛋白呈阳性反应。
